Which of the following will more likely lower stringency conditions in the washing step of a hybridization experiment? - ANSWERS-Increase the concentration of salt in the wash solution buffer
3 cerebrospinal fluid (CSF) tubes labeled 1 through 3 have arrived in your clinical laboratory for e...
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Which of the following will more likely lower stringency conditions in the washing step of a
hybridization experiment? - ANSWERS-Increase the concentration of salt in the wash solution
buffer
3 cerebrospinal fluid (CSF) tubes labeled 1 through 3 have arrived in your clinical laboratory for
evaluation. The tubes were numbered in the order in which they were obtained, with #1 being
the first tube collected and #3 being the last tube collected. What is the best explanation for a
macroscopic appearance seen in the CSF? - ANSWERS-traumatic tap
Bone marrow transplantation is a great method used to treat several malignant and benign
cancers, particularly leukemias. Before transplantation (or engraftment analysis), several
polymorphic loci are screened in both the recipient and donor cells. The main goal of this
screening is to - ANSWERS-find at least one or more informative loci between both the donor
and recipient
What is the rate of DNA translation? - ANSWERS-60 nucleotides per second
polymerase is involved in "short-patch base excision repair": - ANSWERS-Pol β
Replication forks, known as origins of DNA replication, are created by this enzyme: -
ANSWERS-Helicase
You could use all of the following methods to test for the the t(9;22) translocation: - ANSWERS-
FISH; Southern Blot; Reverse Transcriptase PCR
This polymerase acts on DNA and produces Messenger RNA: - ANSWERS-RNA Pol II
The bond between a phosphate group and the ribose sugar group in RNA is called which of the
,following? - ANSWERS-Glycosidic Bond
When this enzyme finds nicks or gaps in the sugar-phosphate backbone of DNA, it closes them!
- ANSWERS-Ligase
While at the doctor's office with your father, you overheard his physician tell another physician
that test results came in, confirming the presence of the Factor V Leiden mutation, a mutation
associated with deep venous thrombosis. Which of the following is the mutation your father has:
- ANSWERS-1691G>A
This polymerase is involved in "replicates mitochondrial DNA": - ANSWERS-Pol γ
Which of the following is a source of DNA damage? - ANSWERS-Chemical damage; attack by
water; radiation damage
What assay amplifies the target using a combination of a three-enzyme system? - ANSWERS-
NASBA
Which of the following storage options is optimal for storing isolated DNA for a period greater
than seven years? - ANSWERS--70*C
Of the PCR based methods, which employs a set of primer pairs designed in such a way that
they overlap, but only one will bind to the target in the presences of SNPs? - ANSWERS-
Amplification Refractory Mutation System (ARMS)
This polymerase acts on DNA and produces Ribosomal RNA: - ANSWERS-RNA Pol I
Mutations in the CFTR gene is associated with what disease? - ANSWERS-Cystic Fibrosis
,Which of the following are characteristic of eukaryotic DNA transcription? - ANSWERS-
Multiple
RNA polymerases; TATA-binding protein; Transcription factors
What assay amplifies the target using reverse transcriptase? - ANSWERS-TMA
This PCR method works by generating a signal at the annealing step (i.e. when the probe binds
its target) of the PCR reaction: - ANSWERS-Molecular beacons
What links codons and anti-codons together during DNA translation? - ANSWERS-
Complementary base pairing
Steps in the Southern blot procedure? - ANSWERS-DNA digest with restriction enzymes; gel
resolution of fragments; fragment denaturation
What are the characteristics of the melt curve analysis? - ANSWERS-The melting temperature
of double stranded DNA depends on its base composition; All PCR products for a specific
primer pair should have the same melting temperature. and length
Its discovery shed light on why there is simultaneous, though not continuous, synthesis of DNA
on both leading and lagging strands of DNA - ANSWERS-Okazaki fragments
The enzyme that primes DNA synthesis is: - ANSWERS-DNA primase
What translocation results in synovial sarcoma, a muscle and fibrous tissue cancer? -
ANSWERS-t(18;X)
Which of the following will increase the specificity of a PCR reaction? - ANSWERS-Use nested
PCR; incorporate DMSO or formamide into rxn; use a high fidelity polymerase
, A reverse dot blot is best performed by: - ANSWERS-Immobilizing multiple unlabeled probes
onto the membrane and then allowing one labeled sample to hybridize to the unlabeled probes
What is the rate of mammalian DNA replication? - ANSWERS-50 nucleotides per second
If two parents are heterozygous for an autosomal recessive disease, then their offspring will most
likely follow this gene frequency pattern: - ANSWERS-25% homozygous dominant, 50%
heterozygous, and 25% homozygous recessive
What enzyme is involved in LCR? - ANSWERS-DNA Ligase
Splicing is the process that does which of the following? - ANSWERS-Remove introns and
preserve exons
A parent has an autosomal dominant disorder. What percent chance does this parent have to
pass down this affected gene to his/her child? - ANSWERS-50%
What are some liquid tumors? - ANSWERS-Mantle Cell lymphomal Burkitt's lymphoma; acute
promyelocytic leukemia
A patient with impaired judgment, personality changes, signs of abnormal body movements and
depression comes to the physician's office for a follow-up visit. The physician suspects a
singlegene disorder may be the cause of those clinical manifestations. A blood specimen was then
sent to your clinical laboratory for mutation screening in the Huntingtin gene. Testing with
standard PCR indicates that patient has Huntington Disease, HD. Which of the following would
be consistent with this diagnosis? - ANSWERS-85 CAG repeats in the Huntingtin gene
What is the sequence recognized by poly (A) polymerase? - ANSWERS-AAUAAA
The rate of DNA migration through an agarose gel during electrophoresis depends on these
factors? - ANSWERS-Net charge; size; shape of molecule
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