Southern Blot - ANSWERS-DNA Target
Nucleic acid probe
Purpose: Paternity testing, gene discovery and mapping, mutation analysis and ID, gene structure
Northern Blot - ANSWERS-RNA Target
Nucleic acid probe
Purpose: Determine transcript structure, processing, and gene expression
Western Blot - ANSWERS-Protein Target
Protein probe
Purpose: Protein processing, gene expression
Southwestern Blot - ANSWERS-Protein target
DNA probe
Purpose: DNA binding proteins, gene regulation
Eastern blot - ANSWERS-Protein target
Protein probe
Purpose: Modification of Western blot using enzymatic detection (protein processing, gene
expression), detection of specific agriculturally important proteins
Far-eastern blot - ANSWERS-Lipid target
No probe
,Purpose: Transfer separated lipids to membranes for analysis by mass spectometry
PCR Primers - ANSWERS-Direct DNA synthesis to desired region
Bioinfo: Identity - ANSWERS-Extent to which 2 sequences are the same
Bioinfo: Alignment - ANSWERS-Lining up 2+ sequences to search for maximal regions
of identity in order to assess extent of relatedness/homology
Bioinfo: Local alignment - ANSWERS-Alignment of some portion of 2 sequences
Bioinfo: Multiple sequence alignment - ANSWERS-Alignment of 3+ sequences arranged
with gaps so that common residues are aligned together
Bioinfo: Optimal alignment - ANSWERS-Alignment of 2 sequences with best degree of
identity
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