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BMW moleculen practicum 2 $3.23   Add to cart
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Essay

BMW moleculen practicum 2
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  • Course
  • Moleculen
  • Institution
  • Universiteit Utrecht (UU)

Uitwerking van het practicum eiwitten van de cursus Metabolisme. In het practicum wordt de alcoholdehydrogenase (ADH) activiteit gemeten onder verschillende omstandigheden mbv een spectrofotometer. Ook worden de berekeningen gegeven en uitgelegd.

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Document information

  • April 23, 2018
  • 5
  • 2016/2017
  • Essay
  • Unknown
  • Unknown

Subjects

  • metabolisme
  • eiwitten
  • alcoholdehydrogenase
  • adh
  • nadh
  • absorptiecoëfficiënt
  • enzymactiviteit

Written for

  • Universiteit Utrecht (UU)
  • Biomedische Wetenschappen
  • Moleculen
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Content preview

Het meten van alcoholdehydrogenase (ADH)
activiteit
TIJD IN METING METING METING
SECONDEN 1 2 3
15 0,21 0,301 0,377
30 0,232 0,337 0,428
45 0,274 0,379 0,456
60 0,317 0,406 0,491
75 0,349 0,456 0,53
90 0,382 0,488 0,58
105 0,418 0,525 0,613
120 0,456 0,563 0,651
135 0,497 0,606 0,671
150 0,518 0,625 0,716

Bij meting 2 hebben we de gemeten waarden verhoogd met 0,100 om zo de lijnen in de grafiek te
laten zien.
Bij meting 3 hebben we de gemeten waarden verhoogd met 0,200 om zo de lijnen in de grafiek te
laten zien.



ADH enzymactiviteit
0,75
0,7 y = 0,0025x + 0,3465
R² = 0,9963
0,65
0,6 y = 0,0025x + 0,265
0,55 R² = 0,9976

0,5
Absorptiewaarde




0,45 y = 0,0024x + 0,1695
0,4 R² = 0,9977

0,35
0,3
0,25
0,2
0,15
0,1
0,05
0
0 20 40 60 80 100 120 140 160
Tijd in seconden

Meting 1 Meting 3 Meting 2 Ijklijn1 Ijklijn 3 Ijklijn 2

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