Essay
BMW moleculen practicum 3
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Uitwerking van het practicum eiwitten van de cursus Metabolisme. In het practicum wordt mbv een activiteitsmeting de ADH-activiteit in gezuiverde en niet-gezuiverde preparaten bepaald.
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ADH-zuiveringResultaten
3A. Zuivering van paardenlever ADH
Tabel 1. Gezuiverd leverextract
TIJD IN METING METING
SECONDEN 1 2
15 0,228 0,219
30 0,243 0,243
45 0,26 0,26
60 0,278 0,276
75 0,296 0,295
90 0,315 0,324
105 0,337 0,345
120 0,358 0,354
135 0,379 0,375
150 0,402 0,397
Absorptiwaarden gezuiverd ADH
0,6
y = 0,0013x + 0,3011
0,5 R² = 0,9958
Absorptiewaarde
0,4
0,3
y = 0,0013x + 0,2028
0,2 R² = 0,9966
0,1
0
0 20 40 60 80 100 120 140 160
Eiwitcocentratie in mg/ml
Meting 1 Meting 2 Ijklijn 1 Ijklijn 2
In de grafiek hebben we de absorptiewaarden van meting 2 verhoogd met 0,100. Hierdoor komt
meting 2 boven meting 1 uit en zijn beide lijnen goed zichtbaar.
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