ASCP Molecular Biology Certification Exam 2024

ASCP Molecular Biology Certification Exam 2024 Pyrimidine - Answer ️️ -One carbon ring Cytosine, Thymine, Uracil Purine - Answer ️️ -Two carbon rings Adenine, Guanine How are nucleotides joined together? - Answer ️️ -Condensation to form phosphodiester bond What is the function of mRNA? - Answer ️️ -Carries genetic info out of nucleus Transcript translated to protein What is the function of tRNA? - Answer ️️ -Carries aa to ribosome Anticodon pairs with codon on mRNA strand What is the function of rRNA? - Answer ️️ -part of ribosome structure most abundant RNA coordinated coupling of tRNA to mRNA codons Feedback inhibition - Answer ️️ -Product of pathway is noncompetitive inhibitor Binds to allosteric site to slow down rxn b/c too much product Exonucleases - Answer ️️ -Degrades nucleic acids by removing one terminal nt at a time Cleaves phosphodiester bond at end of chain 5' -- 3' and 3' -- 5' Endonucleases (Prok) - Answer ️️ -Restriction enzymes Cleaves phoshpodiester bonds w/i poly-nt chain Recognition site is palindromic sequence Types I-V ORI sites - Answer ️️ -nt sequence where replication is initiated Topoisomerase I - Answer ️️ -Induces ss breaks Remove DNA supercoils during TXN and DNA replication; for strand breakage during recombination; for chr condensation; and to disentangle intertwined DNA during mitosis