The aim of this practical is to prepare standard solutions by dilution, to calibrate the colorimeter
and then find the concentration of an unknown Cu 2+ solution.
To find the concentration of the unknown Cu 2+ solution, we began by switching on the colorimeter,
and ensured that the filter was set to 680nm (red filter). Six beakers were labelled as: 0.2 ; 0.4 ; 0.6 ;
0.8 ; 1.0. Using the table below as a guide, the Copper II solution and distilled water were both
measured using the pipettes.
Copper II concentration Volume of Copper Volume of distilled water
(moldm-3) (cm3) (cm3)
1.0 10.0 0.0
0.8 8.0 2.0
0.6 6.0 4.0
0.4 4.0 6.0
0.2 2.0 8.0
0.0 0.0 10.0
After filling the six beakers, the colorimeter was set to %Transmission. A cuvette was filled with 0.0
moldm-3 of the solution, while setting the %Transmission to 100%. The other cuvettes were also filled
with the other solutions and were placed into the colorimeter. The %Transmission was recorded for
each of the cuvettes. The 0.0 moldm-3 cuvette was regularly placed back into the colorimeter to
ensure that the %Transmission was still at 100%.
Then another cuvette was filled with the unknown solution - the absorbance and the %Transmission
were measured.
The experiment was repeated – this time, the filter was changed to the 590nm filter (orange filter).
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