MB ASCP Test Molecular Techniques (20-30%) Questions & Answers 2024

MB ASCP Test Molecular Techniques (20-30%) Questions & Answers 2024 What are the 3 types of gel electrophoresis - ANSWER-Agarose Polyacrylamide Pulse Field What is gel electrophoresis - ANSWER-the separation of materials based on size and migration patterns when subjected to electrical forces in a resistive medium. which direction does the DNA flow in gel electrophoresis - ANSWER-due to DNA being negatively charged, it will migrate towards the positive end What determines how far the DNA will travel in a gel - ANSWER-the size of the DNA fragment What factors affect migration of smaples within a gel - ANSWER-buffer concentrations gel make up physical factors time electric current What is agarose gel electrophoresis - ANSWER-most common gel- polysaccharide extracted from seaweed- concentrations are varied with the size of DNA trying to be separated larger DNA size = lower concentrations of agar (.5%=1%) smaller DNA size = higher concentrations (2%-3%) What is Polyacrylamide gel electrophoresis - ANSWER-used for the resolution of very small DNA segments (PAGE). Can detect 1bp differences in segments. Is chemically prepared and catalyzed into the gel