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Samenvatting immunologie leerjaar 2 HLO deel 2
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Samenvatting immunologie leerjaar 2 HLO Nijmegen deel 2
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Samenvatting immunologie 2Les 1
Herhaling:
1
,3 signalen nodig voor T-cel actvate:
3 signalen nodig voor B-cel actvate:
Daarna proliferate en diferenttate
FLOWCYTOMETRIE
● Meet (metry) stroom (foo) van cellen (cyto)
in druppels
– 30.000-40.000 cellen/seconde
● Door lasers te richten op de stroom
– Meten van licht (FSC= foward: groote
vs SSC=side: vorm)
– Fluorescente
2
, Principe:
-vloeistof wordt opgezogen door fowtp
-hydrodynamische focussing
-lazer gericht op stromende vloeistof.
-Één deeltje per keer passeert de lichtstraal.
Hierdoor krijg je lichtverstrooing:
Detectoren
FSC: foroard scater
-achter de lichtstraal, groote
SSC: side scater
-in een hoek met de lichtstraal
complexiteit, granulariteit
[Granulocyten: hebben een hele hoge side scater dmv granulen]
FORWARD SCATTER
SIDE SCATTER
3
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