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Summary Fecalysis

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Laboratory Procedures involved in processing stool specimens

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  • September 13, 2018
  • 14
  • 2017/2018
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FECALYSIS
DIARRHEA
FECAL EXAMINATION  Increasei iaily stool (above 200 n)
 Macroscopic  Increasei liquiiity ani frequency of more than
 Microscopic three tmes per iay
 Chemical Analyses  Major mechanism:
o Detecton of GI Malinnancies lleeiinn o Secretory
o Liver ani liliary Duct Disoriers o Osmotc
o Maliineston Malabsorpton Synirome o Alterei motlity
o Inflammaton
o Causes of Diarrhea ani Steatorrhea
 Detecton ani Iienticaton of lacteria ani STEATORRHEA
Parasite  Increasei fat in stool (>6 n iay)
o Absence of bile salts that assist
WHAT IS A FECES? pancreatc lipase in the breakiown ani
 Human feces is callei as STOOL. subsequent reabsorpton of trinlyceriies
 Faeces Feces is plural of Latn term “FAEX”  Coniitons associatei:
meaninn RESIDUE. o Pancreatc iisoriers:
 It is the waste resiiue of iniinestble materials of  Cystc Fibrosis
an animal’s iinestve tract expellei throunh the  Chronic Pancreatts
anus iurinn iefecaton.  Carcinoma that iecrease the
 MECONIUM is newborn’s irst feces proiucton of pancreatc enzymes
 SCATOLOGY or COPROLOGY is the stuiy of feces  D-Xylose Test:
o Distnnuish steatorrhea in maliineston
PHYSIOLOGY ani malabsorton coniiton
 Bacterial Metabolism: o D-Xylose is a sunar that ioes not neei to
o Proiuces stronn oior ani flatus be iinestei but ioes not to be absorbei
o Lactose intolerance leais to excessive to be present in the urine
nas proiucton o A normal D-Xylose test iniicates
 Small Intestne: pancreatts
o Major site for inal breakiown ani
reabsorpton of compounis
 Digestve Enzymes: SPECIMEN COLLECTION
o Trypsin, Chymotrypsin, Lipase ani Amino  Container:
Peptiase o Any clean, non-breakable, leakproof
container
 The type ani amount iepenis on the type of
test orierei
o For fecal occult blooi, WlCs or
qualitatve fat, only a small specimen is
requirei
o Quanttatve fecal fat analysis  72-
hour specimen
 The technolonist must be aware of
contaminants such as urine, water or paper


MACROSCOPIC EXAMINATION
 Color
 Consistency
 Form

, COLOR
 Color channes results from GI irrenularites ODOR
 lasically iepenis on the pH of the stool ani
A. Brown  Normal
INDOLE ani SKATOLE are the substances that
B. Gray  Intestnal obstructon larium
proiuce normal oior formei by intestnal
C. Red  llooi or Fooi iyes
bacterial fermentaton an putrefacton
D. Black  llooi from upper GIT, iron therapy,
antaciis, or charcoal treatment  A foul oior is causei by ienraiaton of
E. Green  Venetables, biliveriin uniinestei protein ani excessive carbohyirate
intake
CONSISTENCY  Sickly sweet oior is proiucei by uniinestei
 Formei lactose
 Hari
 Watery CHEMICAL EXAMINATION
 Fecal Blood:
FORM o Detecton of colorectal CA
 Cylinirical  Normal o Hemorrhoiis, anal issures, colon polyps,
 Ribbon-like ulceratve colits ani Crohn’s iisease
o Intestnal strictures such as tumor o Melena:
blockane  Larne fecal blooi (50-100 mL i)
 Small, rouni Scybalous o Occult llooi:
o Constpaton  Small amount of fecal blooi (30-50
 lulky ani frothy mL i)
o Steatorrhea
 Hemoglobin - Reducton Method:
 Mucoii
o H2O2 + Iniicator  Oxiiizei iniicator + H2O
o Colits, constpaton
+ Hb (llue-nreen color)
 Indicators: (Most sensitve to least sensitve)
o Benzidine (Carcinonenic)
o Orthotoluidine
o Guaiac (Most common)

OCCULT BLOOD TESTING INTERFERENCE

False-Positve False-Negatve
 Aspirin ani ant-  Vitamin C > 250
inflammatory meiicatons mn i
 Reai meat  Iron supplements
 Horseraiish, raw broccoli, containinn
cauliflower, raiishes, vitamin C
turnips
 Melons
 Menstrual ani
haemorrhoii
contaminaton

OTHER METHODS…
 Hemoquant  Fluorometric test for haemonlobin
ani porphyrin
 Hemoccult ICT (IFOBT)

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