Summary
MBT samenvatting
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Uitgebreide samenvatting van Microbiologische theorie.
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Shannonvanegmond
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BMTwww.hqppjqqakM
,
.
, Wat doe teen PCR
?
DNA wordtverdubbeld 2 kopieein .
,
Vermeer derd ( amplificeert ) een specifier stun DNA
Waaromdoejeeenpcrreactie ?
aanwezigheid gen of dere
•
an sequent ie aanronen
pick :
homozygoot 2 :
heterozygous
promotor gen ,
of ander • gene tisch onderdeelkloneren
Oversight Standaard PCR en analyse
PCR -
aosarogsea
-1
V
The final
product c- UV visalisation
Waar
op is de
PCR gebaseerd ? DNA replica tie in de cel
leading stand
'
3
'
3
c- I I I
si
Primer '
5 l
3
'
polymerase
'
Ozaki 5
fragm -
.
' " l l
3
Prima se imaaut de
5
'
primers
, DNA replicate heart a rich ting
'
het
• '
DNA 5
polymerase beweegt over ran 3 naar
• ' '
maakt nieuw DNA van 5 naar 3
PCR
Doel van de PCR is om specifier DNA in vitro te kopi Eren ( amplificerenl
Waarmeemaakje een PCR react ie specifier ?
Met primers
In de ••
natuur :
prima se
Vers chill en tasse n replicate versus per
1111111111 -
Replica tie PCR
Helicase
gboc
RNA primers Artificieie DNA primers C
oligo 's )
37°C t Pola 72°C met Tag Pol
↳
groeit in
geiser
Wat
hebjenoaig
?
PCR machine template DNA nucleotide butter
• °
n a
o
.
react ie raatje
.
2 primers
.
Tag .
analyse system
, Forward en Reverse primers DNA polymerase begin
Nucleotide n bouwstenen DNA
K Cl monovalent cation ( let ) voor optimal e binding
Tris buffer voor optimal e pH
Mg Cl
,
divalent Cation ( Mgk ) als cofactor
DNA polymerase enzym dat DNA repliceert
102 -
IOS Kopi een DNA
Erzitten s Stapp en in een PCR
denaturatie ( smelter )
}
uit
°
"
.
annealing ( hybrid isatie primer )
" R '
Y " us
extensie C
elongate ran polymerase ,
°
Staph b.
tap 2 Strap 3
Elke cyclusrerdubbelt het PCR product .
Nancy di
hebje an zoveel material
↳ hang t at ran de groote .
↳
20 dat alle PCR product en die
Nog niet klaarzyn ooh
afgemaakt
Kann en Worden
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