MB - ASCP Review Exam With Correct Questions and Answers Updated 2024 A+ Graded 100%. Her2/neu exhibits an overexpression, what would this indicate? - ANSWER-More aggressive growth and metastasis of the tumor cells in human breast cancer.

MB - ASCP Review Exam With Correct Questions and Answers Updated 2024 A+ Graded 100%. Her2/neu exhibits an overexpression, what would this indicate? - ANSWER-More aggressive growth and metastasis of the tumor cells in human breast cancer. Predominant form of DNA - ANSWER-Right-handed B DNA and histone interaction is due to what type of bond? - ANSWER-Ionic What stains to the minor groove of DNA duplex? - ANSWER-SYBR Green What is the most abundant type of RNA? - ANSWER-Ribosomal RNA (rRNA) What does RNA act as in a retrovirus? - ANSWER-Genome and mRNA What increases half-life of mRNA? - ANSWER-5' Methyl Cap What kind of DNA is most likely to be transcriptionally active? - ANSWER-Euchromatin What is the most common type of eukaryotic DNA? - ANSWER-Satellite DNA What specimen has the most DNA according to molecular weight? - ANSWER-White Blood Cells What amino acids compose histones? - ANSWER-Lysine and Arginine Amino acid that all proteins start with: - ANSWER-Methionine What protects the DNA strand during replication? - ANSWER-Single-stranded binding protein Leading Strand - ANSWER-3' to 5' template Lagging Strand - ANSWER-5' to 3' template What DNA strand is being copied in transcription? - ANSWER-Antisense Strand First step after newly formed mRNA is made? - ANSWER-Addition of poly(A) tail Every time the chromosome is copied it would become shorter if not for what enzyme? - ANSWER-Telomerase What polymerase has repair activity? - ANSWER-Beta/Epsilon What polymerase acts on leading strand? - ANSWER-Delta What polymerase acts on lagging strand? - ANSWER-Alpha Which polymerase is used for mitochondrial replication and repair? - ANSWER-Gamma What polymerase binds the 5' end to 3' end of dsDNA? - ANSWER-Topoisomerase Which RNA polymerase is mainly for transcription? - ANSWER-RNA Pol II Which polymerase is involved with mRNA synthesis? - ANSWER-RNA Pol II What inactivates RNAses? - ANSWER-Diethyl pyrocarbonate (DEPC) or bake glassware for 4 to 6 hours at 400C What type of restriction enzyme recognizes palindromic sites? - ANSWER-Restriction Enzyme Type II What type of restriction enzyme lacks methylation activity? - ANSWER-Restriction Enzyme Type II What is necessary to make cDNA? - ANSWER-Reverse transcriptase Number of SNPs in a human genome? - ANSWER-11 billion (10^7) How many genes (base pairs) are in a human haploid genome? - ANSWER-3 billion base pairs The polymerization of acrylamide requires: - ANSWER-Catalyst (APS or TEMED) or Light activation How does agarose gel pore size relate to concentration? - ANSWER-Inversely proportional What is denatured during a southern blot? - ANSWER-Hydrogen bonds (inter-strand bonds) Melting temperature formula - ANSWER-Tm = (4C x GC Pairs) + (2C x AT pairs) Which is likely to interfere with fluorescent probe? - ANSWER-5' G Proper GC content of primer - ANSWER-35-65% Inhibitors of PCR - ANSWER-Hemoglobin, Heparin, Xylene, Phenol, Cyanol, and Bromophenol Blue Order to add to a PCR reaction: - ANSWER-Master mix patient sample positive control negative control Pre Area - ANSWER-Positive Pressure Post Area - ANSWER-Negative Pressure Which PCR requires two steps for specificity? - ANSWER-Nested PCR Which microorganism is detected by branched DNA amplification? - ANSWER-DNA or RNA Which technique uses RNA, antibodies, and DNA? - ANSWER-Hybrid Capture Assay Use of bisulfite treatment of DNA to determine its pattern of methylation - ANSWER-Bisulfite DNA What tests are isothermal? - ANSWER-NASBA, TMA, & SDA Which leukemia is associated with FLT3 gene? - ANSWER-AML T-cell gene rearrangement is seen in what malignancy? - ANSWER-Sezary Syndrome Which translocation causes Acute Premyelocytic Leukemia? - ANSWER-t 15;17 Ewing Sarcoma - ANSWER-t 11;22 Synovial Sarcoma - ANSWER-t X;18 Follicular Lymphoma - ANSWER-t 14;18 What genes would be screened in a colorectal panel? - ANSWER-MSH2 & MLH1 What gene mutation causes a high risk of breast or ovarian cancer? - ANSWER-BRCA1 and BRCA2 What does MSI test for? - ANSWER-Mutations in mismatch repair genes (MMR) What disease is related to factor V coagulation gene (F5 1q23)? - ANSWER-Thrombossis How many copies are in a full Fragile X mutation? - ANSWER-200-2000+ CGG repeats What disease is known for numerous mutations and large deletions? - ANSWER-Muscular Dystrophy (Duchenne) What HPV causes cervical cancer? - ANSWER-16 & 18 You receive a paraffin embedded specimen for HPV typing, which method should you use? - ANSWER-PCR