Unit 13 - Understand and Interpret Animal Behaviour and Communication
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task 2, 1 Unit 13 - Understand and Interpret Animal Behaviour and Communication
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Unit 13 - Understand and Interpret Animal Behaviour and Communication
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PEARSON (PEARSON)
task 2, 1 Unit 13 - Understand and Interpret Animal Behaviour and Communication (THIS WILL SHOW UP IN THE MARK BOOK AS PLAGIARISM AND YOU WILL GET EXCLUDED OUT THE COURSE) THIS WORK IS MEANT TO BE AS A REFERENCE OR GUIDANCE FOR YOU'RE WORK PLEASE RESPECT MY WORK AND MAKE SURE YOU GIVE ME CREDIT OR...
1 unit 13 understand and interpret animal behaviour and communication
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BTEC
PEARSON (PEARSON)
Animal Management 2010 QCF
Unit 13 - Understand and Interpret Animal Behaviour and Communication
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Task 2, 1
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Task 2 Carry out and write up the following experiment:
1, Chromatography to separate sugars
What chromatography to separate sugars is:
Employing filter-paper chromatography a convenient method (Horizontal Migration Method)
has been developed for the identification of reducing sugars, when present either alone or in
mixtures.
Aim:
The purpose of applying chromatography which is used as a method of quantitative analysis
apart from its separation, is to achieve a satisfactory separation within a suitable time
interval. Various chromatographic methods have been developed to that end. Some of them
include column chromatography, thin-layer chromatography (TLC), paper chromatography,
gas chromatography, ion exchange chromatography, gel permeation chromatography, high-
pressure liquid chromatography, and affinity chromatography
Materials:
● Paper : Usually whatman No. 1 filter paper is used because of its known
● Solvents: [a] Water-saturated phenol + 1% ammonia [b] n-butanol-acetic acid-water
(4:1:5 v/v) [c] isopropanol-pyridine-water-acetic acid (8:8:4:1 v/v)
● Spray reagent: Ninhydrin
● Beaker: 250ml
● Pencil,Ruler
● TLC plate
● Clingfilm
● Oven
● Hair dryer
● Fume cupboard
● Capillary tubes
● 3 different sugars, Glucose, Galactose and Maltose, and a Mixture.
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