TLC Lab Report 5 Study Guide Rated A+
Thin Layer Chromatography consists of three parts:
The analyte, the stationary phase, and mobile phase.
Match each of these terms to what it was in our experiment.
1. Mobile Phase
2. Analyte
3. Stationary Phase
The dominate reason by which analytes are r...
1). Thin layer chromatography consists of three parts:
the analyte, the stationary phase, and mobile phase.
match each of these terms to what it was in our experiment.
1. mobile phase
2. analyte
3. stationary phase
Ans: 1. the solvent
2. one of the analgesics
3. silica
2). The dominate reason by which analytes are retained on a tlc plate is?
Ans: hydrogen bonding
3). If the distance from the origin (start line figure 10.2) to the middle of a spot is 4.8 cm and
the distance from the middle of the same spot to the solvent front (end line figure 10.2) is
3.4 cm, what is the rf for this compound? round to 2 decimal places without units.
Ans: 0.59 +/- .01
4). Complete the sentences.
1. the least polar compound should have a(n)
2. the most polar compound should have a(n)
Ans: 1. larger Rf on a TLC
2. smaller Rf on a TLC
5). Based on our tlc experiments, which analgesic is least polar?
Ans: Ibuprofen
6). The least polar analgesic has...
Ans: a lower ratio of exposed polar to non-polar bonds.
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