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Samenvatting Veilig microbiologische technieken (VMT) Assessment voorbereiding
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Via deze samenvatting van al de theorie van VMT haal je een goede cijfer!! Ik heb met deze samenvatting een 8,1 gehaald!!
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Inhoudsopgave7 soorten bacteriën herkennen..............................................................................................................2
Gramkleuring..........................................................................................................................................5
Werktafel inrichten.................................................................................................................................6
Voedingsbodems....................................................................................................................................6
Kiemgetal................................................................................................................................................6
Reincultuur.............................................................................................................................................7
Gevoeligheid...........................................................................................................................................7
Theorie...................................................................................................................................................8
1. Micro-organismen...........................................................................................................................8
2. Risico’s..........................................................................................................................................10
3. Risicobeheersing...........................................................................................................................10
4. Isoleren.........................................................................................................................................11
5. Risicoklassen.................................................................................................................................14
6. Inperking.......................................................................................................................................15
7. Reinigen en afvalverwerking.........................................................................................................18
8. Incidenten.....................................................................................................................................20
9. Biosafety and biosecurity..............................................................................................................20
,7 soorten bacteriën herkennen
1. Micrococcus luteus
- Bol, gram positief (paars)
- In groepjes van 4
- Kolonie: kleine, bol, glad, citroengeel (kanariegeel/sterk geel), ruikt naar een stal van
dieren
- Mineralisator (in boden omzetting van organische stof tot anorganische), komt voor in
onze omgeving. Niet pathogeen
2. Staphylococcus epidermidis
- Bol, gram positief (paars)
- Druiventrosjes
- Kolonie: matig groot, minder bol, glanzend, glad, creme-wit (niet heel sterk wit), ruikt
zoals 1
- Huidcommensaal: weinig pathogeen
, 3. Staphylococcus aureus
- Bol, gram positief (paars)
- Druiventrosjes, een beetje dicht bij elkaar maar goed te zien.
- Kolonie: matig groot, minder bol, glanzend, glad, goudgeel (beige kleur), geur sterker
dan 1 en 2
- Slijmvliezen van neus- en keelholte. Ontstekingen, abcessen, steenpuisten.
Ontstekingen van been, beenmerg, luchtwege, middenoor, borst, huid, slijmvlies e.d.
4. Enterococcus faecalis
- Bol, gram positief (paars)
- In groepjes van twee
- Kolonie: klein, kleurloos (wit doorzichtig), dezelfde geur, kleine bolletjes , relatief
weinig kolonies
- Darmcommensaal, urineweginfecties, nierbekkenontsteking
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