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Samenvatting Hoofdstuk 3
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Samenvatting hoofdstuk 3van microbiologie.
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Hoofdstuk 3: Bacterieel metabolism3.1 Eukaryoot metabolism
• Een netwerk van door enzyme gekatlyseerde chemische reactes
Katabolisme (afraakreactess
Anabolisme (opbouw- en biosynthese reactess
Minder diversiteit bij eukarytote
celademhaling
3.2 Eukaryoot metabolisme
Katabolisme:
• Aërobe respirate: ( zuurstofadamhalingsprocess hef 3 fasen:
• glycolyse
• Citroenzuurcyclus
• oxidateve fosforylate finale eooeene
electronenacceptor = O2
• Anaërobe fermentate: ALLEEN glycolyse optreedt
endogene elektronenacceptor: pyruvaat melkzuur bij dieren/ ethanol bij planten
Fermentate (eistneg
Zonder zuurstof!
Geen citroenzuurcyclus en geen oxidateve
fosforylate
Alleen GLYCOLYSE
Producte ATP (slechts 2!s
Producte NADH
Producte pyruvaat
PROBLEEM: regenerate NAD+!
è OPLOSSING: mbv pyruvaat verschillende metabolieten
Heterotroof: vertrekkend vanuit organische c-verbindingen waarbij oxidate en het afreken van
deze molecuul optreedt.
Autotroof : vertrekkend vanuit CO2 waarbij reducte en het reduceren van organische c-
verbinding gebeurt.
3.2 Bacterieel metabolisme
Bacteriën:
• Veel diverser dan eukaryoten!!!
• Zijn levende micro-organismen op plekken met:
• pH= 0, pH =13, > 100°C, 0 °C, 5% NaCl
, • Zijn masaal in menselijk lichaam aanwezig
- 10 x meer bacteriën dan cellen in lichaam
- 3% totale lichaamsgewicht
- 10 000 verschillende soorten
- 50% van faeces = bacteriën
- Slechts 15% kweekbaar
Bacterieel metabolisme
• Aërobe respirate
• Anaërobe respirate!
• Fermentate zeer veel verschillende eindproducten!
Aërobe respirate
Idem als eukaryoten
Reacte: C6H12O6 + 6 H2O + 6 O2 ⇒ 6 CO2 + 12 H2O + 38 ATP + warmte
• Suiker CO2 (geoxideerds
• O2 H2O (gereduceerds
è 38 ATP / glucose (ipv 36s
Anaërobe respirate
Andere eooeene terminale elektronenacceptor (eeen O2g
Vbn:
• Nitraatrespirate:
• NO3- N2
• Denitrificate!
• Sulfaatrespirate:
• SO42- H2S
• Stank + zwarte modder
• Carbonaatrespirate:
• CO2 CH4
• Biogas!
Fermentate
Endogene elektronenacceptor (pyruvaats
Verschillende eindproducten bij verschillende fermentates
Vb: melkzuurfermentatee propionzuurfermentatee butaandiolfermentatee alcoholische fermentate
Repirate vs fermentate
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