Unit 6B
Investigating the effect of pH on enzyme activity using starch and amylase
Start date: 14th Feb 2024
Milestones:
- On the 20th March 2024, there is no lesson that day which is going to impact
the project.
- On the 10th April 2024 or depending on when Eid is there is no lesson.
- All other assignments will impact the project
- Check to see if all equipment that is needed is available.
- Include 3 or more results for the experiment
- Only 3 hours of each lesson so the experiment has to be done in sections.
- Collect results in a table and plot a graph.
- Explain the graph and note down any anomalies.
I have no preliminary tests so I got this from online.
There are no participants included in this experiment.
Completion date: May 2024
Plan
Hypothesis- The pH of the reaction mixture affects the amylase enzyme's ability to
break down starch into simpler sugars. According to our hypothesis, amylase
functions best in a specific pH range; if this range is altered, the enzyme will function
less effectively.
Null Hypothesis: The pH of the reaction mixture does not have a significant effect on
the activity of amylase in breaking down starch. There is no specific pH range where
amylase exhibits maximum activity, and deviations from pH 6-7 do not result in
reduced enzyme activity.
, (SaveMyExams, n.d)
The buffer solution is from pH 2- pH 7 using all.
There needs to be 8 test tubes and a test tube rack.
Marker not permanent.
2 spotting tiles.
2 syringes in cm3
Preliminary testing:
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