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Microbiology Lab 5 (2024/2025 ) A+ grade assured
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Microbiology Lab 5 (2024/2025 ) A+ grade assured
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Microbiology Lab 5For the media used in the citrate test, what is the only source of carbon for the bacteria? -
ANScitrate
What is the pH indicator for the citrate test and colors is it at the respective pH? -
ANSbromothymol blue; green at pH 6.9 and blue at pH 7.6 and above
What type of inoculation is used for the citrate test? - ANSstreaking
What is used in a hydrolysis reaction? - ANSwater is used to split complex molecules
What are the enzymes that catalyze hydrolytic reactions - ANShydrolytic enzymes
What are the types of hydrolytic enzymes? - ANSintracellular (endoenzyme) extracellular
(exoenzyme)
Starch hydrolysis is done by what type of enzyme? - ANSexoenzyme
Casein hydrolysis is done by what type of enzyme? - ANSexoenzyme
Urea hydrolysis is done by what type of enzyme? - ANSintracellular
Gelatin hydrolysis is done by what type of enzyme? - ANSexoenzyme
Since starch is too large to be used by the cell, what must happen first? - ANSstarch must
be broken down into subunits
What are two types of exoenzymes used for the hydrolysis of starch? - ANSexoenzymes
a-amylase and oligo 1,6-glucosidase
a-amylase is used for branched or unbranched hydrolysis? - ANSunbranched
oligo 1,6-glucosidase is used for unbranched or branched hydrolysis? - ANSbranched
Starch agar media is used to test for what? - ANSproduction of exoenzymes a-amylase and
oligo 1,6-glucosidase
What is an indicator of starch? - ANSiodine
Casein is a predominant protein in what? - ANSmilk, responsible for its white appearence
What is casease? - ANSa protease that hydrolyzes casein
How is urea produced? - ANSby the catabolism of proteins and amino acids
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