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Lab Quiz 1-- BIO 250L 2024/2025 A+ grade assured
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Lab Quiz 1-- BIO 250L 2024/2025 A+ grade assured
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Lab Quiz 1-- BIO 250Lwhat is the objective of air exposure? - ANSto demonstrate that microorganism are around
us
define the medium? - ANSliquid or gel designed to expose bacteria
what are the two types if medium and what do they provide? - ANSbroth and agar; they
provide variable growth conditions in the form of growth and stability
what three ways do you handle media? - ANSmake sure that the top is on the bottom; plate
is upside down
make sure to seal the plate with parafilm
store in correct temp for the bacteria
define agar - ANSchemical derived from seaweed that solidifies into a jelly-like substance
and becomes a solid
what are two types of agar? - ANSplates and slants
example of agar? - ANSTSA--- trypticase soy agar
what is the air exposure procedure? - ANSopen the plate to expose it to the air for several
minutes then seal with parafilm and place in an incubate at 22C for 24 hours then 4C until
the next week
what is the objective of a surface swab? - ANSto demonstrate that their are microorganism
and can only kill some
what is sterilization and some examples? - ANSwhen you kill all the bacteria;
autoclave and radiation
what is autoclave? - ANSexample of sterilization when you use heat and pressure
what is disinfection and an example? - ANSonly kills some or most of the microorganisms;
Lysol
when streaking plates, be sure to work close to the Bunsen burner why?? - ANSbecause hot
air rises creating a convection current therefore all the bacteria floats up causing your work
no to be contaminated
cross hatch pattern is what and what is it used in? - ANSsurface swab and zigzag long was
and up and down
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