What does CBC stand for? - ANSComplete Blood Count
What are the formed elements of blood? - ANSWBC, RBC, and Platelets
EDTA Anticoagulant - ANSMaintains cell shape and size and prevents blood from clotting by
binding to calcium
Heparin Anticoagulant - ANSInitiates cell clumping and provides blue background staining
Citrate Anticoagulant - ANSDilutes the blood due to the volume of the liquid and is used for
platelet count only
CBC handling and storage: Refrigerate at __ᵒC until testing - ANS4
CBC handling and storage: When transporting to other labs/hospitals - ANSsurround with ice
packs
CBC handling and storage: Samples are _____ before testing - ANSmixed well by inversion
CBC handling and storage: Samples must be tested within __ - ANS24h
CBC handling and storage: Peripheral blood smears must be made within - ANS24h
CBC handling and storage: Samples kept for longer than ___ = CANNOT BE USED FOR
TESTING - ANS24h
What happens if CBC is tested after 24h? - ANSPlatelets clump, WBC nuclei disintegrate, WBC
cytoplasm contain dark granules and Vacuoles, and RBCs swell and break down
Coagulation: Specimen of choice? - ANSPlasma
Coagulation: What colour tube? What is the additive? - ANSlight blue, sodium citrate
Sodium Citrate binds to ___ preventing ____ - ANScalcium, coagulation
Sodium Citrate tube must be - ANSfilled to the maximum draw capacity
Coagulation: Document if patient on - ANSanticoagulant therapy or aspirin
, Handling and Storage of Coagulation samples: Preparation requirements - ANSCentrifuge and
separate
Handling and Storage of Coagulation samples: ____ plasma if not tested within ___ of arrival -
ANSFreeze, 4h
Coagulation samples: Some clotting factors are considered to be - ANShighly labile (destroyed
at room temperature)
What are the three methods of Peripheral blood smears (PBS)? - ANSWedge method (manual),
Spun method (automated slide spinner), and automated method (analyzer)
Thickness of film: HEMOGLOBIN/HEMATOCRIT INCREASED = angle of the spreader slide ___
- ANSDecreased
Sodium Citrate tubes should be drawn ___ except when blood cultures are ordered. - ANSFirst
Thickness of film: HEMOGLOBIN/HEMATOCRIT DECREASED = angle of the spreader slide
___ - ANSIncreased
Good blood film should be ___ to ___ the length of the slide - ANS2/3, ¾
Good blood film should have a slightly ___ or ____ edge - ANSRounded, feathered
The angle of the spreader slide is approximately ___ degrees - ANS35
The greater the angle of the spreader slide the ____ and _____ the film - ANSShorter, Thicker
The lower the angle of the spreader slide the ___ and ____ the film - ANSLonger and Thinner
Blood film should be allowed to ____ ___ completely before staining procedures - ANSair dry
Fixative found in stain is - ANSMethanol
Slides are washed with buffer of pH ___ to develop colour on the slide - ANS6.4
Stain is comprised of ____ and ____ dyes - ANSBasic, acidic
What is the basic (cationic) dye? - ANSMethylene blue
Methylene blue stains the ___ of the cell and the granules of _____ (____ in colour) -
ANSNucleus, basophils, blue-grey
The benefits of buying summaries with Stuvia:
Guaranteed quality through customer reviews
Stuvia customers have reviewed more than 700,000 summaries. This how you know that you are buying the best documents.
Quick and easy check-out
You can quickly pay through credit card or Stuvia-credit for the summaries. There is no membership needed.
Focus on what matters
Your fellow students write the study notes themselves, which is why the documents are always reliable and up-to-date. This ensures you quickly get to the core!
Frequently asked questions
What do I get when I buy this document?
You get a PDF, available immediately after your purchase. The purchased document is accessible anytime, anywhere and indefinitely through your profile.
Satisfaction guarantee: how does it work?
Our satisfaction guarantee ensures that you always find a study document that suits you well. You fill out a form, and our customer service team takes care of the rest.
Who am I buying these notes from?
Stuvia is a marketplace, so you are not buying this document from us, but from seller Ascore. Stuvia facilitates payment to the seller.
Will I be stuck with a subscription?
No, you only buy these notes for $11.99. You're not tied to anything after your purchase.