Thesis
Eiwitkloneringsonderzoek MSH2 MBO
- Course
- Institution
Een volledig uitgewerkt verslag met de benodigde sequenties, achtergrond informatie, kloneringsstrategie en referenties
[Show more]
Seller
Follow
elisereadsbigbookselise
Content preview
MSH2 (DNA Mismatch RepairProtein)
Datum: 03-11-20
Naam: Elise Sugrim
Studentnummer: 1120009
Klas: LM2G
Docent: Kitty Verzijlbergen
, MBO peptide letter: E
MBO plasmide nr: 14
I Achtergrond informatie
Achtergrond
I.1 Peptiden alignen & eiwitsequentie
De verkregen aminozuursequentie is KQIKLDSSAQFGYYFRVTCKEEKVLRNNKNFSTVDIQKN (bijlage 1). Er
is een Protein Blast1 gedaan en bevindt zich in de coderende aminozuursequentie van DNA mismatch
repair protein Msh2 isoform 12. Het accessienummer is NP_000242.12 en het accessienummer van
het mRNA is NM_000251.33.
Er bestaat ook een DNA mismatch repair protein Msh2 isoform 2, maar dit eiwit is niet relevant bij dit
onderzoek.
"MAVQPKETLQLESAAEVGFVRFFQGMPEKPTTTVRLFDRGDFYT
AHGEDALLAAREVFKTQGVIKYMGPAGAKNLQSVVLSKMNFESFVKDLLLVRQYRVEV
YKNRAGNKASKENDWYLAYKASPGNLSQFEDILFGNNDMSASIGVVGVKMSAVDGQRQ
VGVGYVDSIQRKLGLCEFPDNDQFSNLEALLIQIGPKECVLPGGETAGDMGKLRQIIQ
RGGILITERKKADFSTKDIYQDLNRLLKGKKGEQMNSAVLPEMENQVAVSSLSAVIKF
LELLSDDSNFGQFELTTFDFSQYMKLDIAAVRALNLFQGSVEDTTGSQSLAALLNKCK
TPQGQRLVNQWIKQPLMDKNRIEERLNLVEAFVEDAELRQTLQEDLLRRFPDLNRLAK
KFQRQAANLQDCYRLYQGINQLPNVIQALEKHEGKHQKLLLAVFVTPLTDLRSDFSKF
QEMIETTLDMDQVENHEFLVKPSFDPNLSELREIMNDLEKKMQSTLISAARDLGLDPG
KQIKLDSSAQFGYYFRVTCKEEKVLRNNKNFSTVDIQKNGVKFTNSKLTSLNEEYTKN
KTEYEEAQDAIVKEIVNISSGYVEPMQTLNDVLAQLDAVVSFAHVSNGAPVPYVRPAI
LEKGQGRIILKASRHACVEVQDEIAFIPNDVYFEKDKQMFHIITGPNMGGKSTYIRQT
GVIVLMAQIGCFVPCESAEVSIVDCILARVGAGDSQLKGVSTFMAEMLETASILRSAT
KDSLIIIDELGRGTSTYDGFGLAWAISEYIATKIGAFCMFATHFHELTALANQIPTVN
NLHVTALTTEETLTMLYQVKKGVCDQSFGIHVAELANFPKHVIECAKQKALELEEFQY
IGESQGYDIMEPAAKKCYLEREQGEKIIQEFLSKVKQMPFTEMSEENITIKLKQLKAE
VIAKNNSFVNEIISRIKVTT"
I.2 Correcte gennaam + cDNA sequentie + ensembl of NCBI transcript ID
of accession nummer
Het gennaam is MSH22. Ook bekend als COCA1; FCC1; hMSH2; HNPCC; HNPCC1 en LCFS2. Het DNA
mismatch repair protein Msh2 isoform 1 ook bekend als Homo sapiens mutS homolog 2 (MSH2),
transcript variant 1. Dit is het cDNA en het CDS is bruin gemarkeerd (bijlage 2). Het accessienummer
is NM_000251.33.
1 gcattttctt caaccaggag gtgaggaggt ttcgacatgg cggtgcagcc gaaggagacg
61 ctgcagttgg agagcgcggc cgaggtcggc ttcgtgcgct tctttcaggg catgccggag
121 aagccgacca ccacagtgcg ccttttcgac cggggcgact tctatacggc gcacggcgag
181 gacgcgctgc tggccgcccg ggaggtgttc aagacccagg gggtgatcaa gtacatgggg
241 ccggcaggag caaagaatct gcagagtgtt gtgcttagta aaatgaattt tgaatctttt
301 gtaaaagatc ttcttctggt tcgtcagtat agagttgaag tttataagaa tagagctgga
361 aataaggcat ccaaggagaa tgattggtat ttggcatata aggcttctcc tggcaatctc
421 tctcagtttg aagacattct ctttggtaac aatgatatgt cagcttccat tggtgttgtg
481 ggtgttaaaa tgtccgcagt tgatggccag agacaggttg gagttgggta tgtggattcc
541 atacagagga aactaggact gtgtgaattc cctgataatg atcagttctc caatcttgag
601 gctctcctca tccagattgg accaaaggaa tgtgttttac ccggaggaga gactgctgga
661 gacatgggga aactgagaca gataattcaa agaggaggaa ttctgatcac agaaagaaaa
721 aaagctgact tttccacaaa agacatttat caggacctca accggttgtt gaaaggcaaa
781 aagggagagc agatgaatag tgctgtattg ccagaaatgg agaatcaggt tgcagtttca
841 tcactgtctg cggtaatcaa gtttttagaa ctcttatcag atgattccaa ctttggacag
901 tttgaactga ctacttttga cttcagccag tatatgaaat tggatattgc agcagtcaga
961 gcccttaacc tttttcaggg ttctgttgaa gataccactg gctctcagtc tctggctgcc
The benefits of buying summaries with Stuvia:
Guaranteed quality through customer reviews
Stuvia customers have reviewed more than 700,000 summaries. This how you know that you are buying the best documents.
Quick and easy check-out
You can quickly pay through credit card or Stuvia-credit for the summaries. There is no membership needed.
Focus on what matters
Your fellow students write the study notes themselves, which is why the documents are always reliable and up-to-date. This ensures you quickly get to the core!
Frequently asked questions
What do I get when I buy this document?
You get a PDF, available immediately after your purchase. The purchased document is accessible anytime, anywhere and indefinitely through your profile.
Satisfaction guarantee: how does it work?
Our satisfaction guarantee ensures that you always find a study document that suits you well. You fill out a form, and our customer service team takes care of the rest.
Who am I buying these notes from?
Stuvia is a marketplace, so you are not buying this document from us, but from seller elisereadsbigbookselise. Stuvia facilitates payment to the seller.
Will I be stuck with a subscription?
No, you only buy these notes for $5.92. You're not tied to anything after your purchase.
Can Stuvia be trusted?
4.6 stars on Google & Trustpilot (+1000 reviews)
67096 documents were sold in the last 30 days
Founded in 2010, the go-to place to buy study notes for 14 years now