Principes van GEE: Lambda PCR , Kolonie PCR, Plasmide digestie en Gelelektroforese
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Course
B03GEE
Institution
Hogeschool Leiden (HSL)
Ontvang een complete PowerPoint-presentatie voor GEE! Deze presentatie behandelt essentiële technieken zoals Lambda PCR, Kolonie PCR, Plasmide Digestie en Gelelektroforese.
Principes van GEE
Module B03GEE
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, Lambda PCR principe
• DNA vermeerderen
• Mastermix
• Target DNA
• Primers
• DNA polymerasen
• dNTP-mix nucleotides
Stap Tijd Temperatuur
• PCR-buffer
Denatureren 4 min 94 °C
• Cofactor Mg2+
Denatureren 30 sec 94 °C
• Optimale omstandigheden Annealing 30 sec 55 °C
• Gelelektroforese principe Extensie 1 min 72 °C
Verlengde Extesie 10 min 72 °C
Koelen 5 min 55 °C
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