Principes van GEE: Lambda PCR , Kolonie PCR, Plasmide digestie en Gelelektroforese
9 views 0 purchase
Course
B03GEE
Institution
Hogeschool Leiden (HSL)
Ontvang een complete PowerPoint-presentatie voor GEE! Deze presentatie behandelt essentiële technieken zoals Lambda PCR, Kolonie PCR, Plasmide Digestie en Gelelektroforese.
Principes van GEE
Module B03GEE
Gemaakt door
Studentnummer
Klas
Datum
Praktijkdocent
, Lambda PCR principe
• DNA vermeerderen
• Mastermix
• Target DNA
• Primers
• DNA polymerasen
• dNTP-mix nucleotides
Stap Tijd Temperatuur
• PCR-buffer
Denatureren 4 min 94 °C
• Cofactor Mg2+
Denatureren 30 sec 94 °C
• Optimale omstandigheden Annealing 30 sec 55 °C
• Gelelektroforese principe Extensie 1 min 72 °C
Verlengde Extesie 10 min 72 °C
Koelen 5 min 55 °C
The benefits of buying summaries with Stuvia:
Guaranteed quality through customer reviews
Stuvia customers have reviewed more than 700,000 summaries. This how you know that you are buying the best documents.
Quick and easy check-out
You can quickly pay through credit card or Stuvia-credit for the summaries. There is no membership needed.
Focus on what matters
Your fellow students write the study notes themselves, which is why the documents are always reliable and up-to-date. This ensures you quickly get to the core!
Frequently asked questions
What do I get when I buy this document?
You get a PDF, available immediately after your purchase. The purchased document is accessible anytime, anywhere and indefinitely through your profile.
Satisfaction guarantee: how does it work?
Our satisfaction guarantee ensures that you always find a study document that suits you well. You fill out a form, and our customer service team takes care of the rest.
Who am I buying these notes from?
Stuvia is a marketplace, so you are not buying this document from us, but from seller vergonets. Stuvia facilitates payment to the seller.
Will I be stuck with a subscription?
No, you only buy these notes for $7.69. You're not tied to anything after your purchase.