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Samenvatting Les 1: methoden en technieken in de histologie
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Samenvatting van les 1 uit de online Sofia cursus van prof. Frederik De Smet.
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InhoudsopgaveMethoden en technieken in de histologie ....................................................................................................... 2
1. Inleiding .................................................................................................................................................... 2
1.1. Wat is weefselleer? ......................................................................................................................... 2
1.2. Verschillende microscopische technieken ...................................................................................... 2
1.3. De resolutie van microscopen ......................................................................................................... 3
1.4. Het gebruik van licht- en elektronenmicroscopie in de histologie .................................................. 3
1.5. Anatomopathologie ........................................................................................................................ 4
2. Weefselcollectie voor histologie ............................................................................................................... 4
3. Elektronenmicroscopie.............................................................................................................................. 5
3.1. Analyse van ultrastructuren ............................................................................................................ 5
3.2. Transmissie-elektronenmicroscopie (TEM) ..................................................................................... 5
3.3. Staalpreparatie voor TEM ............................................................................................................... 5
3.4. Stalen ‘kleuren’ voor TEM ............................................................................................................... 5
3.5. Scanning elektronenmicroscopie .................................................................................................... 6
4. Lichtmicroscopie ....................................................................................................................................... 6
4.1. Weefselpreparatie voor lichtmicroscopie ....................................................................................... 6
4.2. Weefselpreparatie voor lichtmicroscopie (2).................................................................................. 6
4.3. Weefselbewaring en fixatie............................................................................................................. 7
4.4. Weefselbewaring en fixatie (2) ....................................................................................................... 8
5. Weefselkleuringen voor histologie............................................................................................................ 8
5.1. Staalpreparatie voor kleuringen...................................................................................................... 8
5.2. Haematoxyline-eosine kleuring....................................................................................................... 8
5.3. Empirische kleuring ......................................................................................................................... 9
5.4. Histochemische kleuring ................................................................................................................. 9
5.5. Enzymhistochemische kleuring ....................................................................................................... 9
5.6. Immunohistochemische kleuringen .............................................................................................. 10
5.7. Immunohistochemische kleuringen (2)......................................................................................... 11
5.8. Immunohistochemische kleuringen (3)......................................................................................... 11
2018-2019 1
, Weefselleer: les 1
Methoden en technieken in de histologie
1. Inleiding
1.1. Wat is weefselleer?
Cel = basis functionele eenheid van een weefsel
Uit de structuur ervan kan de functie worden afgeleid: vorm volgt functie
Met het blote oog waarneembaar: anatomie
1.2. Verschillende microscopische technieken
Microscoop = instrument voor het bestuderen van objecten, die te klein zijn om goed met
het blote oog te kunnen worden gezien
A) Lichtmicroscoop
Gebruikt makend van zichtbaar licht
Stereomicroscoop: licht dat op het object zelf invalt en weerkaatst
“gewone” microscoop: licht dat door valt
Oculairen en objectieve zijn verwisselbaar
2018-2019 2
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