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applied science - chemistry- unit 19 c part 2 - distinction level

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Task 2: How to identify analytes using GC and HPLC

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  • May 14, 2024
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  • 2023/2024
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Task 2: How to identify analytes using GC and HPLC

State the key features of a GC chromatogram and what information can be obtained from one.

The retention time is the time taken for a component of a compound to travel though the column. It
helps to identify compound

The peak shape can indicate if the separation was efficient. Long and narrow suggest good
separation while broad and short suggest bad separation

Peak area can be used to know the quantity of compound in a sample. This is useful for analysis

Peak width can indicate if the separation was efficient or not. long and narrow suggest good
separation while broad and short suggest bad separation.

Baseline is the detectors response to no compound. Stable and flat baseline can suggest a clean
chromatogram



State the key features of a HPLC chromatogram and what information can be obtained from one.



The retention time is the time taken for a component of a compound to travel though the column. It
helps to identify compound

The peak shape can indicate if the separation was efficient. Long and narrow suggest good
separation while broad and short suggest bad separation

Peak area can be used to know the quantity of compound in a sample. This is useful for analysis

Peak width can indicate if the separation was efficient or not. ong and narrow suggest good
separation while broad and short suggest bad separation.

Baseline is the detectors response to no compound. Stable and flat baseline can sugest a clean
chromatogram

, GC Chromatograms.

I will be analysing 4 different chromatograms from against a reference chromatogram to determine
the pesticides present, the peak area of each sample and thus the percentage of each analyte
present and the concentration of each analyte in the sample.

 Explain how to calculate peak area. Include an example calculation.

To calculate the peak are you must measure the heigh of the peak and multiply it by the half of the
width. it cam be represented as;

Height of peak/ hath of the width of peak

Example

Height is 15

Width is 4

The it will be

7 /2 = 3.5

3.5 is the peak area

 Explain how to calculate percentage of each analyte. Include an example calculation.

To calculate the percentage of each analyte, it must be the peak area of the analyte divided by sum
of all the peak area.

It can be represented as

peak area/sum of all the peak area x100

example

you have an beak areas of 34,7.8,15,10,16.8

what you will do is to add up every thing which will give you 83.6

then you use the sum to divide by the individual peak area and multiply by 100

(34/83.6) x100 = 14.67%




 Explain how to calculate concentration of each analyte. Include an example calculation




To calculate the concentration of each analyte

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