Essay
applied science - chemistry- unit 19 c part 2 - distinction level
- Course
- Institution
Task 2: How to identify analytes using GC and HPLC
[Show more]
1 review
By: cliodhnafagan97 • 5 months ago
Seller
Follow
chiam
Reviews received
Content preview
Task 2: How to identify analytes using GC and HPLCState the key features of a GC chromatogram and what information can be obtained from one.
The retention time is the time taken for a component of a compound to travel though the column. It
helps to identify compound
The peak shape can indicate if the separation was efficient. Long and narrow suggest good
separation while broad and short suggest bad separation
Peak area can be used to know the quantity of compound in a sample. This is useful for analysis
Peak width can indicate if the separation was efficient or not. long and narrow suggest good
separation while broad and short suggest bad separation.
Baseline is the detectors response to no compound. Stable and flat baseline can suggest a clean
chromatogram
State the key features of a HPLC chromatogram and what information can be obtained from one.
The retention time is the time taken for a component of a compound to travel though the column. It
helps to identify compound
The peak shape can indicate if the separation was efficient. Long and narrow suggest good
separation while broad and short suggest bad separation
Peak area can be used to know the quantity of compound in a sample. This is useful for analysis
Peak width can indicate if the separation was efficient or not. ong and narrow suggest good
separation while broad and short suggest bad separation.
Baseline is the detectors response to no compound. Stable and flat baseline can sugest a clean
chromatogram
, GC Chromatograms.
I will be analysing 4 different chromatograms from against a reference chromatogram to determine
the pesticides present, the peak area of each sample and thus the percentage of each analyte
present and the concentration of each analyte in the sample.
Explain how to calculate peak area. Include an example calculation.
To calculate the peak are you must measure the heigh of the peak and multiply it by the half of the
width. it cam be represented as;
Height of peak/ hath of the width of peak
Example
Height is 15
Width is 4
The it will be
7 /2 = 3.5
3.5 is the peak area
Explain how to calculate percentage of each analyte. Include an example calculation.
To calculate the percentage of each analyte, it must be the peak area of the analyte divided by sum
of all the peak area.
It can be represented as
peak area/sum of all the peak area x100
example
you have an beak areas of 34,7.8,15,10,16.8
what you will do is to add up every thing which will give you 83.6
then you use the sum to divide by the individual peak area and multiply by 100
(34/83.6) x100 = 14.67%
Explain how to calculate concentration of each analyte. Include an example calculation
To calculate the concentration of each analyte
The benefits of buying summaries with Stuvia:
Guaranteed quality through customer reviews
Stuvia customers have reviewed more than 700,000 summaries. This how you know that you are buying the best documents.
Quick and easy check-out
You can quickly pay through credit card or Stuvia-credit for the summaries. There is no membership needed.
Focus on what matters
Your fellow students write the study notes themselves, which is why the documents are always reliable and up-to-date. This ensures you quickly get to the core!
Frequently asked questions
What do I get when I buy this document?
You get a PDF, available immediately after your purchase. The purchased document is accessible anytime, anywhere and indefinitely through your profile.
Satisfaction guarantee: how does it work?
Our satisfaction guarantee ensures that you always find a study document that suits you well. You fill out a form, and our customer service team takes care of the rest.
Who am I buying these notes from?
Stuvia is a marketplace, so you are not buying this document from us, but from seller chiam. Stuvia facilitates payment to the seller.
Will I be stuck with a subscription?
No, you only buy these notes for $9.31. You're not tied to anything after your purchase.
Can Stuvia be trusted?
4.6 stars on Google & Trustpilot (+1000 reviews)
76799 documents were sold in the last 30 days
Founded in 2010, the go-to place to buy study notes for 14 years now