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Samenvatting Medische microbiologie Examen juni 2024
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Samenvatting medische microbiologie Bevat - gram positieve bacteriën - Serologie - Mycobacterium - Antibiogram
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MEDISCHE MICROBIOLOGIE :EXAMEN JUNI 2024
Lector. Van de Velde Brigitte
Bevat :
• Gram positieve bacteriën
• Mycobacterium
• Serologie
• Antibiogram
2023-2024
,INHOUD
1. Gram positieve bacteriën .................................................................................................................................... 2
1.1 Gram positieve kokken .................................................................................................................................. 2
1.1.1 Staphylococcus ....................................................................................................................................... 2
1.1.2 Streptococcus ......................................................................................................................................... 4
1.1.3 Enterococcus .......................................................................................................................................... 7
1.1.4 Zeldzaam voorkomende gram positieve kokken.................................................................................... 7
1.2 Gram positieve staven................................................................................................................................... 7
1.2.1 Aëroob en facultatief aëroob ................................................................................................................. 7
1.2.2 Strikt anaërobe ..................................................................................................................................... 11
2. Mycobacterium tuberculosis = Zuurvaste bacteriën......................................................................................... 13
2.1 Info uit powerpoint ..................................................................................................................................... 13
2.2 Te kennen werkcolleges 6 en 7 ................................................................................................................... 14
2.3 MC ............................................................................................................................................................... 15
3. Serologie ........................................................................................................................................................... 16
3.1 Agglutinatie technieken .............................................................................................................................. 16
3.2 Immunofluorescentie .................................................................................................................................. 17
3.3 Interpretatie van de resultaten ................................................................................................................... 17
3.4 Te kennen werkcollege 8............................................................................................................................. 18
4. Antibiogram ...................................................................................................................................................... 19
4.1 Biologische gevoeligheid (MIC) ................................................................................................................... 19
4.2 Klinische gevoeligheid ................................................................................................................................. 21
1
Moons N / Vermeulen A
, 1. GRAM POSITIEVE BACTERIËN
Gram positieve kokken Gram positieve staven
• Staphylococcus (Facultatief) aëroob Strikt anaëroob
• Streptococcus • Corynebacterium • Clostridium
• Enterococcus • Listeria • Actinomyces
• Zeldzaam voorkomend • Lactobacillus • Propionibacterium
• Erysipelothrix
• Rhodococcus
• Nocardia
• Bacillus
• Garnerella
1.1 GRAM POSITIEVE KOKKEN
1.1.1 STAPHYLOCOCCUS
Indeling
• Staphylococcus aureus → bevat : MRSA en CA-MRSA
• CNS → = coagulase negatieve stafylokken
S. AUREUS VS CNS
Staphylococcus aureus CNS
Voorkomen Huid, keel en neus Commensaal huid en slijmvliezen
→ merendeel bevolking is drager
Pathogeen BELANGRIJKE PATHOGEEN GERINGE PATHOGEEN
vermogen → binnen en buiten ziekenhuis → binnen ziekenhuis (nosocomiaal)
• Huidinfecties • Kathetersepsis
o Furunkel • Endocarditis (hartklep)
o Impetigo → baby’s • Prothese infecties
• Pneumonie
• Sepsis
• Metastatische abcessen
• Toxisch shock syndroom
• Enterocolitis
Opm : Opm :
Produceert coagulase, toxine,… Bepaalde CNS produceren slijmkapsel
→ werkt infecties in de hand → hechten zo vast op vreemd materiaal
Labo-diagnose
• Gramkleuring Trosvormig gram Ꚛ kokken Trosvormig gram Ꚛ kokken
• Isolatie
o Bloed agar Goudgeel & -hemolyse Witte kolonies & GEEN -hemolyse
o MSA Geel & mannitol Ꚛ (afbreken) Mannitol Θ (geen afbraak)
• Identificatie
o Katalase + +
o Coagulasetest + -
o DNA’se + -
o Aëroob facultatief facultatief
2
Moons N / Vermeulen A
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