Unit 10 - Biological Molecules and Metabolic Pathways
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UNIT 10B APPLIED SCIENCE UP TO DISTINCTION
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Unit 10 - Biological Molecules and Metabolic Pathways
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Unit 10: biological molecules and metabolic pathways Learning aim b: explore the factors that can affect respiration. BTEC Level 3 Extended Diploma in Ap...
Unit 10 - Biological Molecules and Metabolic Pathways
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Unit 10 B: practical investigation Investigation:
Introduction:
We performed this experiment in groups of three and timed the
length of time it took for the bromothymol blue solution to turn
from blue to green. To determine how long it would take for the
bromothymol solution to turn green, we had to blow into a straw
filled with the solution while timing ourselves with a timer. To
acquire reliable data, we had to repeat this procedure several
times after engaging in an exercise like running up and down
stairs to observe how quickly the solution changed colour in
response to an increase in heart rate. As expected, the solution
changed colour more quickly than usual.
Independent variable:
Exercise
Dependant variable:
The amount of time it takes for the bromothymol blue solution to
turn blue to yellow green.
Control variables:
Volume of water and bromothymol blue solution, temperature,
time of day, and, gender, and health status of participants.
Hypothesis:
The time taken for the bromothymol blue solution to change
colour will be shorter after exercise, indicating that exercise
increases the rate of breathing and the production of carbon
dioxide.
Equipment:
• Measuring cylinder
• Bromothymol blue
• 50ml beakers/test tubes and test tube rack
• Water
• Straws
• Stopwatch
, Health and safety risks:
Participants should be healthy and able to perform the exercise
safely. The beakers should be handled carefully to avoid
breakage or spillage of the solution. Bromothymol blue is not
toxic, but it can cause skin and eye irritation if it meets these
areas. Therefore, it is advisable to wear gloves and goggles when
handling the solution. Finally, it is important to dispose of the
solution properly after the experiment is complete, as it can be
harmful to the environment if it is not disposed of correctly.
Method:
• Make sure to precisely measure the 40 ml of water and 10 ml
of bromothymol solution before adding them to the 10 ml
beaker.
• Put a straw in the beaker, then get out your stopwatch.
• Blow through the straw until the solution becomes green
after you've set the timer, then note how long it took.
• After finishing this, rinse the beaker and repeat the
procedure, but this time you must exercise. Once your heart
rate has risen, you must blow into the straw and keep track
of the time until the solution becomes green once more.
• You could choose between a variety of different exercises
like running up and down the stairs and doing star jumps.
• You should repeat this experiment several times to get
accurate results.
Conclusion:
Exercise accelerates breathing and increases carbon dioxide generation, according to
the findings of this investigation. activity speeds up the generation of carbon dioxide,
as seen by the substantially faster time it took for the bromothymol blue solution to
turn yellow-green following activity. It should be mentioned that there are some
restrictions on this experiment. The experiment doesn't fully capture the process of
breathing and carbon dioxide production; it simply measures a single aspect of it.
Individual variations in breathing patterns and lung capacity may also have an impact
on the experiment, which could decrease the precision of the findings.
Despite these limitations, this experiment, is still useful for
educational purposes. It can help students understand the
relationship between exercise, breathing, and carbon dioxide
production. Further research may be needed to confirm these
,findings and to explore the relationship between exercise and
carbon dioxide production in detail.
Analysis:
The project aims to investigate how breathing and carbon dioxide
production are affected by exercise. The experiment involves
timing how long it took for a bromothymol blue solution to turn
blue to yellow green both during and after exercise. In the
experiment, it was discovered that exercise considerably
shortened the time it took for the bromothymol blue solution to
change colour, indicating a rise in carbon dioxide production.
The experiment was well designed, and the results were reliable.
The use of a bromothymol blue solution to measure carbon
dioxide production is a standard method used in many
experiments, and the experiment followed appropriate safety
procedures.
Evaluation:
The experiment had some limitations. The experiment only
measured one aspect of breathing and carbon dioxide production
and may not be representative of the whole process.
Additionally, the experiment may be affected by individual
differences in breathing patterns and lung capacity, which may
limit the accuracy of the results. Overall, the experiment was
successful in demonstrating the relationship between exercise,
breathing and carbon dioxide production. The experiment was
well
designed, and the results were reliable. The experiment is a
useful education tool, and further research may be needed to
explore the relationship between exercise and carbon dioxide
production in more detail.
Strengths:
This experiment is relatively easy to perform and can be done
with minimal equipment. It provides a visual representation of
the effect of exercise on breathing and carbon dioxide
production.
, Weaknesses:
The experiment does not provide a quantitative measure of
carbon dioxide production, and it may be affected by individual
differences in breathing patterns and lung capacity.
Advantages:
The experiment is easy to understand and can be used to teach
students about the relationship between exercise and carbon
dioxide production.
Disadvantages:
Due to individual variations in breathing patterns and lung
capacity, the experiment may not be precise enough to be used
for scientific research.
Limitations:
The experiment has limitations because it only examines one
component of breathing and carbon dioxide production and could
not be an accurate representation of the entire procedure.
Effectiveness:
The experiment is effective for educational purposes and can
help students understand the relationship between exercise,
breathing and carbon dioxide production. However, it may not be
accurate enough for scientific research.
Secondary data:
Physical Trial 1 Trial 2 Trial 3 Average
activity
Rest 130 128 132 130
Walking 115 120 112 115.6
slowly
Power 93 91 98 216.6
walking
Running at a 43 31 38 37.3
steady pace
Sprinting on 10 8 6 8
a
treadmill
Bicep curls 65 58 63 62
Dead lift 57 48 47 50.6
,My results:
Running on the My data 1 minute and 30
spot seconds
I conducted this experiment to better understand how exercise
changes the level of carbon dioxide in the breath. This
experiment shows the time it takes for the solution to transition
from blue to green or yellow following your workout. After the
experiment was finished, the time it took for the solution to
change colour was quicker than the amount of carbon dioxide
generated. The experiments' two results were precise.
Factors Affecting Respiration
Description (BP4):
1. Chemicals in Cigarettes:
Cigarette smoke contains a myriad of harmful chemicals that can
wreak havoc on the respiratory system. These chemicals can be
broadly categorized into toxins, tar, and nicotine.
a. Toxins: Cigarette smoke is a complex mixture of over 7,000
chemicals, many of which are toxic. One of the most dangerous
toxins is carbon monoxide (CO). CO has a higher affinity for
hemoglobin than oxygen, causing it to bind to hemoglobin and
form carboxyhemoglobin. This reduces the oxygen-carrying
capacity of the blood, leading to hypoxia (low oxygen levels) in
tissues and organs.
,Other toxins in cigarette smoke include hydrogen cyanide,
formaldehyde, and benzene. These toxins can damage lung
tissue, impair lung function, and increase the risk of respiratory
infections and diseases.
b. Tar: Tar is a sticky, particulate matter in cigarette smoke that
can coat the respiratory tract. It contains numerous carcinogenic
compounds, such as polycyclic aromatic hydrocarbons (PAHs) and
tobacco-specific nitrosamines (TSNAs). Tar can cause
inflammation and irritation of the respiratory tract, leading to
conditions like chronic bronchitis and lung cancer.
c. Nicotine: While not directly damaging to the respiratory
system, nicotine is a highly addictive substance that can have
indirect effects on respiration. Nicotine stimulates the release of
epinephrine (adrenaline), which can constrict blood vessels,
including those in the lungs. This can reduce the efficiency of gas
exchange and increase the workload on the respiratory system.
2. Drugs:
Certain drugs, both legal and illegal, can have detrimental effects
on respiration.
a. Ketamine: Ketamine is a dissociative anesthetic that can
depress the respiratory system. At high doses, it can cause
respiratory depression, where the rate and depth of breathing
are significantly reduced. In severe cases, this can lead to
respiratory arrest, a life-threatening condition where breathing
stops altogether.
b. Cocaine: Cocaine is a powerful stimulant that can constrict
blood vessels, including those in the lungs. This can lead to
pulmonary hypertension, a condition characterized by high blood
pressure in the arteries that supply the lungs. Pulmonary
hypertension can cause shortness of breath, chest pain, and, in
severe cases, heart failure.
,3. Pollutants:
Environmental pollutants can have a significant impact on
respiratory health.
a. Asbestos: Asbestos is a naturally occurring fibrous mineral
that was widely used in construction and insulation materials due
to its heat-resistant properties. However, inhaling asbestos
fibers can cause serious respiratory problems. These fibers can
become lodged in the lungs, causing inflammation and scarring, a
condition known as asbestosis. Asbestos exposure is also a
leading cause of mesothelioma, a rare and aggressive form of
cancer that affects the lining of the lungs and chest cavity.
b. Oxidants: Oxidants are reactive molecules that can damage
lung tissue through oxidative stress. Common oxidants include
ozone, nitrogen dioxide, and particulate matter (PM) from
sources like vehicle emissions and industrial processes. Exposure
to oxidants can exacerbate respiratory conditions like asthma
and chronic obstructive pulmonary disease (COPD).
4. Respiratory Diseases:
Certain respiratory diseases can directly impact the efficiency of
respiration.
a. Asthma: Asthma is a chronic inflammatory condition that
affects the airways. During an asthma attack, the airways
become constricted, inflamed, and filled with mucus, making it
difficult to breathe. Asthma attacks can be triggered by various
factors, including allergens, exercise, respiratory infections, and
environmental pollutants.
b. Chronic Obstructive Pulmonary Disease (COPD): COPD is a
progressive lung disease that includes chronic bronchitis and
emphysema. In COPD, the airways become inflamed and
narrowed, and the air sacs (alveoli) in the lungs can become
damaged or destroyed. This makes it increasingly difficult for the
, lungs to take in oxygen and expel carbon dioxide, leading to
shortness of breath and other respiratory problems.
Explanation (BM3):
Cigarette Smoke and Its Constituents:
In addition to tar, nicotine, and carbon monoxide, cigarette
smoke contains thousands of other chemicals that can be harmful
to the respiratory system. These include hydrogen cyanide,
formaldehyde, benzene, and radioactive compounds like
polonium-210.
Tar is particularly damaging because it contains many
carcinogenic substances like polycyclic aromatic hydrocarbons
(PAHs) and tobacco-specific nitrosamines (TSNAs). When tar
particles are inhaled, they can initiate the formation of DNA
adducts, which are alterations in DNA that can lead to genetic
mutations and ultimately cancer development. The carcinogens
in tar put smokers at high risk for lung cancer as well as cancers
of the larynx, oral cavity, and esophagus.
The inflammation caused by tar and particulate matter exposure
also attracts inflammatory cells like neutrophils to the lungs.
These cells release proteolytic enzymes that can destroy the
connective tissue in the alveoli, leading to emphysema. Loss of
alveolar surface area significantly reduces the capacity for gas
exchange.
Nicotine causes vasoconstriction by activating the sympathetic
nervous system and releasing neurotransmitters like
epinephrine. This not only reduces pulmonary blood flow but can
also contribute to pulmonary hypertension over time as the
vessels remodel and thicken.
Carbon monoxide's disruption of oxygen transport is
compounded by the fact that it can remain bound to hemoglobin
for an extended period due to its high affinity. This "storage"
effect means hypoxia persists long after smoke exposure ends.
Asbestos Fibers:
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