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Assignment 19c
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Essay of 18 pages for the course unit 19 at PEARSON (DISTINCTION LEVEL)
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Assignment 19c10665368
Bushra Habib
Gas Chromatography
A method that separates chemical components of a specific example mixture
is called Gas Chromatography. To detect components this method makes
sure of the presence or absence of components. There are two phases called
stationary and mobile phase. Gas that does not react is called mobile phase
but is also known as the carrier gas in the picture below. The liquid that
layers the surface of the material which is solid is known as stationary phase.
Thermal stability and volatility are needed for a component to be analyzed.
Functions of each part of this diagram:
Carrier Gas
This plays the role of carrying the sample through the chromatographic
column. It aids the process of separation of the individual components
that are in the samples by moving them forward through the column at
different speeds depending on their interactions with the materials of
the column.
Proportioning Valve
This has control over the carrier gas and samples flow into the column
of chromatography. It makes sure that constant and accurate sample
, proportions are being injected into the column for the process of
separation and analysis.
Flow Controller
The function of this is to balance the rate of flow of the carrier gas in
the gas chromatography. It makes sure of an accurate and constant
flow rate of the carrier gas through the column of chromatography, this
is vital for precision of separation and the analysis of components in
the sample.
Sample Injector
This makes the sample meet the system of gas chromatography. It
accurately sends small samples amounts into the stream of carrier gas,
this enables it to be moved through the column of chromatography for
analysis and separation.
Detector
Its job is to detect the components that have been separated as elution
occurs from the chromatograph column. It starts off the signals which
correlate with each component's concentration, this allows them to be
identified and quantified.
Gas Chromatography
This causes separation in the sample's components depending on their
interactions towards the stationary phase in the column. This is a very
important part of the system of chromatography where the compounds
separate depending on their indifferences for the carrier gas and
stationary phase. The type, diameter and length of the columns
stationary phase confirms the efficiency of separation and the analysis’
selectivity.
Column Oven
This looks over and takes care of the temperature constancy. This
control of temperature is needed for separation, which is reproducible,
this has an impact on interaction which occurs between stationary
phase and components of the sample. The column makes sure of
constant analytical results when the temperature in it is kept stable
and accurate.
Gas System
, This gathers, processes and analyses the signals that take place by the
detector. It changes the signals to chromatograms; these are
representations of graphs of the components that separate over some
time. In addition, the system involves software like data interpretation,
peak integration, quantifications, and result reporting.
1
At the retention time of 34.19 the compound of Licoflavanone was found, a,
at the retention time of 45.20 the compound of Pinocembrin was found and
at the retention time of 54.30 the compound of Glabranin was found in this
GC Chromatogram. The retention time of each compound is different.
YOU CAN CALCULATE THE CONCENTRATION OF A COMPOUND WITH THE USE
OF A CHROMATOGRAM BY:
Peak identification – find the peak on the graph that correlates with the
compound that is being analyzed.
Measure the area of peak – by the use of a software or manual
procedures to confirm the area that lies under the peak. That area
shows the compound amount that is present in that graph.
1
NO HARVARD REFERENCING FOUND FOR THIS IMAGES LINK
https://www.researchgate.net/figure/Gas-chromatography-mass-spectrometry-GC-MS-
chromatograms-of-the-phytocomplexes-from_fig2_333908419
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