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Samenvatting Belangrijkste afleidingen, grafieken, tekeningen en pathways voor Industriële Microbiologie (De Vuyst) $8.81   Add to cart

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Samenvatting Belangrijkste afleidingen, grafieken, tekeningen en pathways voor Industriële Microbiologie (De Vuyst)

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Dit zijn de belangrijkste afleidingen, grafieken, tekeningen en pathways voor Industriële Microbiologie van prof. De Vuyst

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  • May 24, 2024
  • 74
  • 2022/2023
  • Summary
avatar-seller
17 cursus)
Industriele
Kurken
Microbiologie
microbiele celles
Na
:
van

Biomana
groeit it mee




·
-




afstering Substraats
Biomanax Cx
=




↑ =
Cs
Primairemetabolieten P Cp =




Secundaine metabolieten-coiolieten
-




Enzymen-Ce
> t




Inoculum felten op t =
o wantleven ontstaat uitbeven ,
exponentie Gia
Groei afnemen
openaktzatodepanluad
Substraat
WanneerOn
--
:




hi e
no
popase In d
-

:




Substraat = C-bron N-bron P-bron
, , ,
S-bron


za
(n)
Fumentatie A bio conversieprocessem
.




Fermentatie : ES + Xo - Xo + AX + EPI Cellen
spelen eer rol!


Bioconcessie : S-P Door
enzymen
(it
clafh)
(15) Celnitrienten
ps
:




Algemene Formule X (Biomana) :
CHrs00s Ho . a




ps(29) chelaatiomes
:




Carboxyl coo Amino- NHL
Merapto -SH
. :
:
-




1

, Overzicht
ps
(13) var d
stram downstam
processtappes van en
:
-




frmentalproductieproces
--




UpstiamMO

fumentation
Sauces of C, N, P S
, ,
raw

minos element
materiale
, ta elements , GFF,

als isolatie
init ..
water ↓
development

media
I
strain
improvement
&

propagatic ↓ ↓maintenance
medium
production thair medium




S
Constraints nutritional
requirements,
productie Supported suzunde
:



a

metabolic controls shean

medium thfumental en
-




sensitivity



,
, -
&


ik
temp optima morfologie elected
-
OaxCO2
trumentmodeofgratietariation
geometry
, ,



requirements genetic stability metabolic
,


,




byproducts viscosity /fects

witantie
,




> starter culture -
↳ elementor
propagatie Aimentativ
SPA
koeling verwarming
Downstram
influenced byproducteach Step
A ⑪
excitupI
in Situ D
i
Hilde
stability
<




all I
purity requirement separation
casta
process
filte
Centrifugation/
!
alion
-
I
-




bioman-havested cells &
' Y
medium
·waste ↓
:
spentd Pim
! auf
ifproduct
extracellular
intracellular
vi
extracellular I
recovery
periprarmicprod. product
,
I
I

↓ e
concentration
I I


all
dimption
!
I


d I



I




"
--
alldebui antie
alle
d
-
Jugation/
afiltration
-



~
I

I
I
cell-f inclusion
I




I us medem
exhact bodies concentrate
-



S I
I
V
-- ↓ - - - -




Thematografi s che , --
-
- -

-




Product
precyptatie artition stappen i unification
- -




!- Dialyse , & ,




ultraf
-




ithatie distill
, ---





--------------
-----
- - -




fini
-




Kistallisatie
drogen byof
ilisatie
, , shing ,
-- fillatie verpakken--- processes
-




steriele
-




/


- V
-




effluent Iinished product

2

,pa(52) Michaelis-Menten : kinetiek

SEP
ks
A
E + S = ES ⑱
*4
E + P
d kz & ↳


enzym
subthaat
product
>
-
↳ moet zo klein
mogelijk zijn ,
anders maak
jeGeen product/it maximaal
k
km = >
- Michaelis Menter - te


·

Vergelijking MM van : v : reactiesnelheid (Mmol/h) 253
MM
:



MM
g
~=
Naas of v =
Rg(t
num
+ ]
. met Kr =
kaaks
k1



1
d Umax
max reactiesnelheid

lineaire
igl
Queenkomende
snelheidug/gintegree MMig substraat
->




= E Cs


↑ Na product Cx =




alti por antzi
verbruiktS


Grafische postelling MMig Typerbolische
o
..
care


A
besit
er - - - - - - - -


Asymptotische wijse ~max
: alle
enzymen zijn


X
wat

met substraat

1
Grote Pri trage
-
*




Place
ma

Km snellen
-




:




..

dit stuk
tikt
en rechte
op
↳ [S] reactiekinetick
Umax als

E
v
= " onde



v =
~max als [S] km Dode "




=> MM
plat lincasser zie
volgen



3

, pa(53)
Buck
i Lineuraver
plat
= max
u
1 ma

1/v N




-Km/vmax

max

- >
1/C


a) Eadie-
Hofste plot
v=
-


knv +
Vmax
Es
v


-
M




-antinen w



3)
Hane-Wolf (Langmin) plat
= max
max

CINN
↓Numax

e >s
KM
-




4) Fadie-Scatchard
plat
= -M

N
u/s


ema -
- se




4

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