Exam (elaborations)
BMSC 210 Midterm 2 | 100% Correct Answers | Verified | Latest 2024 Version
- Course
- Institution
BMSC 210 Midterm 2 | 100% Correct Answers | Verified | Latest 2024 Version
[Show more]
Content preview
BMSC 210 Midterm 2 | 100% Correct Answers | Verified | Latest 2024 Version What is genetic engineering? - ✔✔using in vitro techniques to alter genetic material in the lab What is recombinant DNA technology? - ✔✔the artificial recombination of DNA from two organisms What are some basic techniques of genetic engineering? - ✔✔ DNA amplification electrophoesis nucleic acid hybridization molecular cloning expressing foreign genes targeted mutagenesis What is heterologous expression? - ✔✔expressing a gene in a different host What is the polymerase chain reaction? - ✔✔rapid amplification in the number of copies of specific DNA sequences for further analysis What is a thermocycler? - ✔✔an automated PCR machine What is quantitative PCR? - ✔✔quantifies initial amount of DNA What are some applications of PCR? - ✔✔-obtain specific fragments of DNA for cloning -comparative (phylogenic) studies to amplify genes and DNA sequences -amplify VERY small amounts of DNA (forensic) What are the steps in PCR amplification? - ✔✔1. DNA is denatured by heating 2. Synthetic piece of DNA flanking sequence of interest is added 3. Add DNA polymerase (Taq or Pfu polymerase) 4. heat and cool - repeat Why is use of thermostable DNA polymerase critical? - ✔✔because of high temperatures What do PCR require? - ✔✔DNA polymerase and artificial oligonucleotide primers made of DNA What is reverse transcription PCR? - ✔✔can make DNA from an mRNA template What enzyme is used in reverse transcription PCR? - ✔✔reverse transcriptase - converts RNA into complementary DNA What is gel electrophoresis? - ✔✔the separation of charges molecules using an electric field What are agarose gels stained with? - ✔✔ethidium bromide DNA cut with different restriction enzymes will have the same or different banding patterns on an agarose gel? - ✔✔different What is nucleic acid hybridization? - ✔✔Single strands of DNA, or of DNA and RNA, from related organisms will hydrogen bond to form a double stranded molecule What are nucleic acid probes? - ✔✔ss DNA fragment complementary to the gene of sequence of interest How are nucleic acid probes created? - ✔✔cloning, synthesis, denaturing a fragment of DNA What is a southern blot? - ✔✔a hybridization procedure where DNA is in the gel and probe is RNA or DNA What is a northern blot? - ✔✔RNA Is in the gel and the probe is DNA or RNA What is FISH? - ✔✔Fluorescence in situ hybridization (FISH) uses fluorescent probes attached to oligonucleotide What is molecular cloning? - ✔✔movement of a gene from original source to small genetic element (vector) which can be manipulated What are the main steps of gene cloning? - ✔✔1. isolation and fragmentation of source DNA 2. Insertion of DNA fragment into cloning vector 3. Introduction of cloned DNA into host organism What are restriction endonuclease enzymes? - ✔✔tool in genetic engineering that recognizes specific DNA sequences and cut DNA What do type II restriction enzyme endonucleases do? - ✔✔-cleaves DNA within recognition sequence -most useful for specific DNA manipulation What do type I restriction enzyme endonucleases do? - ✔✔-first discovered + purified -cuts DNA at random for from recognition sequence What do type III restriction enzyme endonuclease do? - ✔✔-large, combination restriction and modification enzymes - cleaves outside of recognition sequences Structure of cleaved product from type II REases may differ how? - ✔✔3' or 5' overhang and blunt endsThe benefits of buying summaries with Stuvia:
Guaranteed quality through customer reviews
Stuvia customers have reviewed more than 700,000 summaries. This how you know that you are buying the best documents.
Quick and easy check-out
You can quickly pay through credit card or Stuvia-credit for the summaries. There is no membership needed.
Focus on what matters
Your fellow students write the study notes themselves, which is why the documents are always reliable and up-to-date. This ensures you quickly get to the core!
Frequently asked questions
What do I get when I buy this document?
You get a PDF, available immediately after your purchase. The purchased document is accessible anytime, anywhere and indefinitely through your profile.
Satisfaction guarantee: how does it work?
Our satisfaction guarantee ensures that you always find a study document that suits you well. You fill out a form, and our customer service team takes care of the rest.
Who am I buying these notes from?
Stuvia is a marketplace, so you are not buying this document from us, but from seller Tulloch. Stuvia facilitates payment to the seller.
Will I be stuck with a subscription?
No, you only buy these notes for $12.49. You're not tied to anything after your purchase.
Can Stuvia be trusted?
4.6 stars on Google & Trustpilot (+1000 reviews)
73314 documents were sold in the last 30 days
Founded in 2010, the go-to place to buy study notes for 14 years now