Summary Exploring Protein Detection: A Guide to Modern Assays
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Course
AU858 (BIOL07013)
Institution
ATU
PDF presentation that summarizes, protein assays and detection methods. Includes the basic steps of an ELISA and why they are required. Alongside types of detection (direct/indirect) as well as types of ELISA (direct, indirect, sandwich, competitive) and Immunohistochemistry uses
Coating of solid phase
with antibody and antigen
Block remaining binding
sites
Add antigen/antibody to
Use a s
be detected
detec
Wash out excess reagent amplificati
, An
Int
Antibodies de
Stronger Ab-A
of lower
Specificity of b
with serum pro
Balancing sen
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