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Samenvatting Moleculaire Biologie Technieken
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Samenvatting Moleculaire Biologie Technieken. Met deze samenvatting heb ik een 7,6 gehaald. De leerdoelen zijn verwerkt in het document. TLSC-MOLBIO3V-16
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By: Joesef • 5 year ago
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InhoudLes 1, Algemene DNA Technieken..........................................................................................................2
Les 2, Algemene DNA Technieken..........................................................................................................4
Les 3, PCR...............................................................................................................................................8
Les 4, Sequencen..................................................................................................................................11
Les 5, Opsporen van Genen en Functiebepaling...................................................................................12
Les 6, CRISPR/Cas.................................................................................................................................14
1
, Les 1, Algemene DNA
Technieken
Kloneren:
Het produceren van genetische identieke
organismen.
Moleculair kloneren:
Het genereren van recombinant DNA en de
replicatie daarvan in gast organismen,
gebruikmakend van moleculair biologische
technieken.
Amplificatie door bacteriën:
Plasmide DNA als kloneervector:
Nieuwe genen genereren, vermenigvuldigen
en tot expressie brengen.
Bevat:
- ORI: bindingsplaats voor DNApol,
startplaats replicatie.
- MCS: unieke knipsites.
- Selectiemarkers.
Recombinant DNA technologie:
Enzymen:
- Nucleases (restrictie-enzymen),
knippen
- Modificatoren, bewerken
- Ligases, plakken
- Polymerases, kopiëren
Nucleases:
- Endo- en exonucleases
- Knipt tussen desoxyribose en
fosfaatgroep.
- Bacterie beschermt zichzelf door A en
C te methyleren.
Restrictie-enzymen:
2
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