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Biol 105 Final Lab Exam IVY Tech.
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Biol 105 Final Lab Exam IVY TechAgar - ANS-solidifying agent for nutrient broth
Allele - ANS-Different forms of a gene
Bacillus - ANS-Rod shaped bacteria
Biogenesis - ANS-the production of living organisms from other living organisms
Centromere - ANS-Region of a chromosome where the two sister chromatids attach
Comma shaped bacteria are ? - ANS-Vibrio
crossing over - ANS-exchange of genetic material between homologous chromosomes
during prophase I of meiosis
Dominant - ANS-An allele that is always expressed
fastidious - ANS-Will not grown well in unsupplemented broth or agar
G1 phase - ANS-metabolic activities, building up and breaking down macro molecules,
this also the phase that initiates mitosis
G2 phase - ANS-Growth and preparation for cell division
Gene - ANS-A segment of DNA on a chromosome that codes for a specific trait
Genotype - ANS-An organism's genetic makeup, or allele combinations.
Gram-negative bacteria - ANS-Bacteria that have a thin peptidoglycan cell wall covered
by an outer plasma membrane. They stain very lightly (pink) in Gram stain.
Gram-negative bacteria are typically more resistant to antibiotics than Gram-positive
bacteria.
Gram-positive bacteria - ANS-Bacteria that have a thick peptido glycan cell wall, and no
outer membrane. They stain very darkly (purple) in Gram stain.
, Gram's iodine does what? - ANS-It is a mordant meaning it helps the stain attach to the
cell better.
heat fixing - ANS-kills the bacteria, makes them adhere to the slide, and coagulates
cytoplasmic proteins to make them more visible
Heterozygous - ANS-An organism that has two different alleles for a trait
Homozygous - ANS-An organism that has two identical alleles for a trait
If the concentration of bacteria is low less than 3000 bacteria per ml how can you
estimate the number of bacteria ? - ANS-by counting the number of colonies that form
after mixing a sample with molten nutrient agar, this is true when the cells do not stick
together
If the concentration of bacteria is over 3000 bacteria per ml how would you determine
the number of bacteria ? - ANS-By performing a serial dilution
Is Ethidium Bromide a safe stain for gel electrophoresis ? - ANS-No it is a carcinogen
M-phase - ANS-The phase of the cell cycle that includes mitosis and cytokinesis.
normal flora - ANS-bacteria which are found in or on our bodies on a semi-permanent
basis without causing disease
nutrient broth - ANS-commonly used liquid complex medium for culture growth
Phenotype - ANS-An organism's physical appearance, or visible traits.
Recessive - ANS-An allele that is masked when a dominant allele is present
S phase - ANS-The synthesis phase of the cell cycle; the portion of interphase during
which DNA is replicated.
spontaneous generation - ANS-the mistaken idea that living things can arise from
nonliving sources
synapsis - ANS-the fusion of chromosome pairs at the start of meiosis.
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