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QUIZ 1 – MSAP EXAM QUESTIONS CORRECTLY SOLVED TO SCORE A+
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QUIZ 1 – MSAP EXAM QUESTIONS CORRECTLY SOLVED TO SCORE A+
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QUIZ 1 – MSAP EXAM QUESTIONS CORRECTLY SOLVED TO SCORE A+OriC Answer - site of replication on the ProK DNA
What 2 important sequences are located on the OriC? Answer - 13-mer in tandem array and 9-mer (there are 4)
What does the DnaA bind? Answer - The 9-mer sequence of DNA on ProK.
What is the significance of the DNA sequences at the OriC? Answer - They are A-T rich at the 13-mer sequences.
The DnaA binds to the 9-mer sequences
What is DnaC? Answer - Helicase inhibitor - delivers DnaB to the template (ProK replication)
What is DnaB? Answer - Helicase (ProK replication) and binds primase to form primosome.
What is DnaA? Answer - initiator proteins, breaks hydrogen bonds (ProK)
single stranded binding protein (ssb) Answer - binds single stranded DNA to prevent re-annealing.
Primase Answer - primes the DNA for the new strand using RNA
What is the primasome? Answer - DnaB and primase
DNA poly III Answer - Polymerase for elongation in a 5'-3' manner. Also has exonuclease in a 3'-5'
DNA poly 1 Answer - exonuclease for the Okasaki fragments, polymerase for the fragments.
Ligase Answer - fixes the nicks
Topoisomerase Answer - corrects the "overwinding" in DNA replication in circular DNA. by nicking the DNA What is sigma factor? Answer - in ProK, it is part of the holoenzyme (included with primary poly).
- only needed to start transcription
What does RNA poly I make? Answer - 5.8S, 18S, 28S rRNA in EuK.
What does RNA poly II make? Answer - mRNA and some snRNA
What does RNA poly III make? Answer - tRNA and some snRNA
What are the regions of the ProK gene? Answer - - promoter
- RNA coding sequence
- terminator
What is the promoter called in ProK for transcription? Answer - -35 and -10 (Pribnow box) consensus sequences
What are the promoters for EuK transcription? Answer - GC box
TATA box
CAAT box
conserved short sequence elements as a collection
TATA box Binding Protein (TBP) Answer - subunit of TFIID
binds to TATA box of gene promoter
TFIID Answer - causes distortion in the DNA helix, allowing recruitment of other factors
TFIIB Answer - start site recognition, involved in RNA poly interactions (EuK)
TFIIH Answer - contains DNA helicase, activates RNA poly by phosphorylation
TFIIE Answer - involved in positioning RNA polymerase
What are the 3 stop codons? Answer - UGA, UAG, UAA
What are the general characteristics of the genetic code? Answer - - triplet code
- no commas
- non overlapping
- universal (almost)
- degenerate
- start and stop codons
what charges a tRNA? Answer - aminoacyl-tRNA synthetase
How is the amino acid activated? Answer - Through linkage of a AMP to its carboxyl group forming aminoacyl-AMP
IF-1 and IF-2 (prok) Answer - come in with GTP and 30S ribosomal unit. leave when large subunit comes in with the help of GTP - change to GDP.
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