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3. ASCP Blood Banking Question Pool
Rh

✅✅
(Warm autoantibodies often exhibit Rh specificity "IgG".) - -Autoantibodies
demonstrating blood group specificity in warm autoimmune hemolytic anemia are
associated more often with which blood group system?

A. Rh
B. I
C. P
D. Duffy

perform a warm autoadsorption

(Since the auto control is positive after the AHG phase and no reactivity was
detected at immediate spin, the serology is most consistent with a warm
autoantibody. An adsorption with autologous cells to remove the antibody and

✅✅
subsequent testing of the adsorbed plasma or serum for alloantibody detection is the
next step.) - -A 26-year-old female is admitted with anemia of undetermined
origin. Blood samples are received with a crossmatch request for 1 unit of Red Blood
Cells. The patient is group A, Rh-negative and has no history of transfusion or
pregnancy. The following results were obtained in pretransfusion testing:
sample IS 37C IAT
cell 1 0 0 3+
cell 2 0 0 3+
autocnt 0 0 3+
donor 0 0 3+

The next step to continue this investigation would be:

6.0

(Determination of compatibility can be determined by multiplying the percentage of

✅✅
compatibility of each antigen. 45% of the population is group O, 15% are D-negative,
and 91% are K-negative. 0.46 × 0.15 × 0.91 = 0.06, or 6%.) - -A patient is
group O, Rh-negative with anti-D and anti-K in her serum. What percentage of the
general Caucasian donor population would be compatible with this patient?

Black

(20% percent of persons of African ethnicity are Group B compared to only 11% of
those of European ethnicity. For the Rh phenotype results, 44% of blacks compared

,to 4% of whites will demonstrate the Dce phenotype.) - ✅✅-The following blood
typing results were noted on a patient's sample:

anti-A: 0
anti-B: 4+
anti-D: 4+
A1 cells: 4+
B cells: 0
Rh typing: C-E-c+e+

What is the patient's likely ethnicity?

2 days - 2 weeks

(Delayed hemolytic transfusion reactions are caused by a secondary anamnestic
response in a previously alloimmunized recipient. Unlike a primary response, a

✅✅
secondary response is rapid. Antibody may be detectable 2 days to 2 weeks from
the time of transfusion.) - -In a delayed transfusion reaction, the causative
antibody is generally too weak to be detected in routine compatibility testing and
antibody screening tests, but is typically detectable at what point after transfusion?

phenotype his cells to determine which additional alloantibodies may be produced


✅✅
(Determining the patient's phenotype allows focusing identification procedures
toward antibodies the patient can develop.) - -A patient received 4 units of
blood 2 years previously and now has multiple antibodies. He has not been
transfused since that time. It would be most helpful to:

M, N

(The M and N antigens are found on glycophorin A.) - ✅✅-Which of the following
red cell antigens are found on glycophorin-A?

A. M, N
B. Lewis a, Lewis b
C. S, s
D. P, P1, Pk

allergic

(Allergic reactions are a type 1 immediate hypersensitivity reaction to an allergen in

✅✅
plasma. Most are mild reactions shown by urticaria "hives, swollen red wheals"
which may cause itching.) - -Hives and itching are symptoms of which of the
following transfusion reactions?

, A. febrile
B. allergic
C. circulatory overload
D. bacterial

group AB, Rh-positive


✅✅
(FFP should be ABO compatible with the recipient's RBCs. Avoid FFP with ABO
antibodies to A or B antigens the patient may have.) - -A patient who is group
AB, Rh-negative needs 2 units of Fresh Frozen Plasma. Which of the following units
of plasma would be most acceptable for transfusion?

A. group O, Rh-negative
B. group A, Rh-negative
C. group B, Rh-positive
D. group AB, Rh-positive

ABO incompatibility

(ABO HDFN occurs most commonly in group A babies born to group O mothers and
usually has a mild course. The DAT is typically weak or negative and jaundice

✅✅
develops 12 - 48 hours after birth. The mother and baby are both Rh-positive.) -
-A group A, Rh-positive infant of a group O, Rh-positive mother has a weakly
positive direct antiglobulin test and a moderately elevated bilirubin 12 hours after
birth. The most likely cause is:

Group O, Rh-negative red cells should be given

(In an emergency situation, group O red cells are given if the patient's ABO and Rh

✅✅
are unknown. In the case of women with child-bearing potential, it is preferential to
provide group O, Rh-negative red cells.) - -In an emergency situation, what
type of blood should be given to a female patient of child-bearing age if the ABO
group and Rh type are unknown?

prevent graft-vs-host (GVH) disease

(Irradiation inhibits proliferation of T cells and subsequent GVHD.) - ✅✅-The
purpose of irradiation of blood components is to:

the weak D test and control would be positive

(A positive DAT will interfere with weak D testing causing both the patient and control
to demonstrate positive results. Any positive result in the control tube invalidates any

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