MLPAO Test Prep ~ Histology and
Cytology
Histology Definition - ANS-Study of the structure of tissues
Histology: Types of specimens received - ANS-Surgical procedures, biopsies, autopsies
Histology: Surgical specimens include - ANS-Whole or part of an organ, cysts, tumors
Histology: Biopsy specimens include - ANS-Removal of a representative sample
(through needle aspiration, surgery, or endoscopy)
Histology: Autopsy specimens include - ANS-Removal of tissue from a deceased
person (to analyze the cause of death, diagnosis, or for research)
Specimens must be labelled with - ANS-First and last name, DOB, pt address, Unique
ID number, OHIP number, Specimen type, Specimen number
Specimens are assigned a ____ number through LIS - ANS-Surgical
Specimens must have a _______ with them - ANS-requisition
Requisition / on-line request must contain the following - ANS-pt first and last name,
DOB, Unique ID Number, OHIP number, pt Address, pt location, ordering physician,
specimen type, specimen number (if there is more than one specimen)
Specimens arrive in the lab as either a ___ or ____ specimen - ANS-Fixed, unfixed
What is the first step when dealing with processing histology specimens? - ANS-Verify
patient information of requisition
Act in which components of cells and tissues are fixed in a physical and somewhat
chemical state to endure treatment, avoiding loss, alteration, and decomposition -
ANS-Fixation
Specimens should be placed in a fixative that is ____ the estimated volume of the tissue
- ANS-10-20X
, What is considered to be the universal fixative? - ANS-Formalin/formaldehyde
Fixation preserves tissue in a - ANS-lifelike manner
Fixation prevents - ANS-putrefaction and autolysis
Autolysis definition - ANS-Decomposition due to the action of enzymes
Putrefaction definition - ANS-Decomposition due to the action of bacteria
Fixation denatures or precipitates ___ - ANS-Proteins
Fixation: Go through beneficial ___ - ANS-hardening
Fixation: Enhances ____ procedures - ANS-staining
Fixation: ____ microorganisms - ANS-fixes
Advantages of other fixatives: B5 - ANS-Displays good nuclear detail
Advantages of other fixatives: Glutaraldehyde - ANS-Used in electron microscopy
Fixative that fixes tissues for cross-linking proteins - ANS-Glutaraldehyde
Grossing of specimens Record the description of tissue including - ANS-Size, color,
consistency, and any abnormalities (if present)
Grossing of specimens tissues are - ANS-Sliced open and viewed
Grossing of specimens biopsies and smaller sample handling - ANS-placed in lens
paper
Decalcification definition - ANS-Controlled removal of calcium from the tissue
Tissue must be ____ before being decalcified - ANS-Fixed
Why must tissue be fixed before decalcification? - ANS-Cell morphology will be
damaged by the strong strength of the decalcification solution
Examples of decalcification agents - ANS-Nitric acid, formic acid, EDTA
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