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This list is limited to media you would use in a medical microbiology lab and some you would use in the food or bio-pharm industries.
All information is displayed in an easy to read, color-...
MEDIA TYPE SAMPLE NOTABLE
NAME WHAT IT DOES
INGREDIENTS TYPES ORGANISMS
Enriched and Almost ALL Streptococcus spp., Grows fastidious organisms
Differential: 5% samples Pseudomonas Produces hemolytic patterns which can
Sheep Sheep Blood get a blood aeruginosa: metallic help ID bacteria
Blood plate: sheen Beta - complete lysing of RBCs on plate
(SBA or wounds, Proteus spp.: swarms Alpha -incomplete or partial lysing.
BAP) eyes, Serratia marcescens: Appears green
throats, brick red Gamma - non-hemolytic. Colonies are
etc. usually large, white, and raised
Enriched: NAD+ Sterile H. influenzae: needs Grows fastidious organisms
(factor V) and sites, both X and V factors CHOC is SBA that has had RBCs lysed to make
Chocolate hemin (factor X) wounds, H. parainfluenzae: nutrients readily available
(CHOC) eyes, ears, needs factor V
sputums H. ducreyi: needs
factor X
Selective: crystal Wounds, Pseudomonas Inhibits gram pos
violet and bile salts ears, aeruginosa: green- Crystal violet and bile salts allow growth of
MacConke sputums, blue. Considered gram neg bacteria. Inhibits gram pos.
y Differential: lactose urines NLF. Contains
(MAC) pyocyanin (pigment Lactose differentiates between lactose
pH indicator: altering fermenters (LF) and non-lactose fermenters
Neutral Red fermentation) (NLF)
Selective: 7.5% Nasal Staphylococcus Inhibits all gram neg and selects for
NaCl swabs only aureus and Staphylococcus spp.
halophiles (salt- Purpose is to ID S. aureus from other non-
Mannitol Differential: loving organisms) pathogenic Staph spp.
Salt Agar Mannitol
(MSA) NaCl inhibits all gram neg and allows growth
pH indicator: of Staph spp.
Phenol Red Mannitol differentiates mannitol fermenters
(MF) from non-mannitol fermenters (NMF)
MEDIA REFERENCE GUIDE
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