Exam (elaborations)
MCB 2010L Exam 2.
- Course
- Institution
Exam of 8 pages for the course Microbiology Ivy Tech BIOL201 at Microbiology Ivy Tech BIOL201 (MCB 2010L Exam 2.)
[Show more]
Seller
Follow
lydiaomutho
Content preview
MCB 2010L Exam 2Name the steps in aseptic transfer of liquid (broth) media. - ANS-Flame the loop with
bunsen burner flame
Grab the broth and swirl it
Flame the tube (Grab it with dominant)
Hold the tube at an angle to prevent contamination
Remove loop from broth
Reflame tube replace cap
Make a smear of bacteria on designation
Sterilize loop with flame
Name the steps in aseptic transfer of a plate media. - ANS-Flame the loop with bunsen
burner flame
Swirl the loop with bacteria transfer to plate use lid as shield
Remove loop from plate
Sterilize loop with flame
Name the steps in aseptic transfer of a slant media. - ANS-Flame the loop with bunsen
burner flame
Flame the tube
Start at base of slant and come up
Transfer to designated tube
Flame the tube once again
Flame the loop after transfer
Which medium was most difficult to determine a type of growth? - ANS-The liquid
medium was the most difficult (broth) because the bacteria was on the bottom
Why in this exercise where specific types of exposure chosen? - ANS-To determine the
different pathogens or bacterias that exist and how we can identify them through either
a test tube or a petri plate.
Free living pathogen - ANS-are free-living; they can survive and reproduce in the
environment without the presence of a host species
What is nonpathogenic bacteria? - ANS-A bacteria incapable of causing disease ie.
e.coli
, What is a pathogen? - ANS-A disease causing agent ie. virus
What is an opportunistic pathogen? - ANS-An infectious microorganism that is normally
a commensal or does not harm its host but can cause disease when the host's
resistance is low
What is a reservoir? - ANS-The reservoir of an infectious agent is the habitat in which
the agent normally lives, grows, and multiplies
What is the definition of ubiquity? - ANS-Something that appears everywhere
Bacillus - ANS-Rod shaped bacteria
Coccus - ANS-Spherical
Spirillium - ANS-spiral shaped bacteria
Vibrio - ANS-comma shaped
Spirochete - ANS-spiral shaped bacteria
What is a pure culture? - ANS-population of cells derived from a single cell
Streptococci - ANS-bacteria that form a chain
Staphylococci - ANS-Pus-forming bacteria that grow in clusters like a bunch of grapes.
They cause abscesses, pustules, and boils.
Streptobacilli - ANS-chains of bacilli
What is a CFU? - ANS--colony forming unit
-only between 30 and 300 cfus are considered statistically valid
What is the purpose of streak plate method? - ANS-isolate pure colonies of bacteria
If you did not obtain isolation in your plate, why would that happen? - ANS-If there are
no colonies, you either did not contaminate correctly or the plate was contaminated with
nearby bacteria.
The benefits of buying summaries with Stuvia:
Guaranteed quality through customer reviews
Stuvia customers have reviewed more than 700,000 summaries. This how you know that you are buying the best documents.
Quick and easy check-out
You can quickly pay through credit card or Stuvia-credit for the summaries. There is no membership needed.
Focus on what matters
Your fellow students write the study notes themselves, which is why the documents are always reliable and up-to-date. This ensures you quickly get to the core!
Frequently asked questions
What do I get when I buy this document?
You get a PDF, available immediately after your purchase. The purchased document is accessible anytime, anywhere and indefinitely through your profile.
Satisfaction guarantee: how does it work?
Our satisfaction guarantee ensures that you always find a study document that suits you well. You fill out a form, and our customer service team takes care of the rest.
Who am I buying these notes from?
Stuvia is a marketplace, so you are not buying this document from us, but from seller lydiaomutho. Stuvia facilitates payment to the seller.
Will I be stuck with a subscription?
No, you only buy these notes for $7.99. You're not tied to anything after your purchase.
Can Stuvia be trusted?
4.6 stars on Google & Trustpilot (+1000 reviews)
72841 documents were sold in the last 30 days
Founded in 2010, the go-to place to buy study notes for 14 years now