PORTAGE MICROBIOLOGY EXAM #3 QUESTIONS WITH COMPLETE SOLUTIONS VERIFIED GRADED A+
What is the total magnification of a sample imaged with a 60x objective and a 10x eyepiece? (short answer)
60 x 10 = 600x magnification
True or False: A cell that is adherent, flat, and unstained is easily identifi...
What is the total magnification of a sample imaged with a 60x objective and a 10x
eyepiece? (short answer)
60 x 10 = 600x magnification
True or False: A cell that is adherent, flat, and unstained is easily identified using
bright field microscopy
False. Adherent, flat cells are almost invisible due to the limits on both resolution and
contrast
Which of the following could be seen clearly by the unaided eye? (multiple
choice)
Protozoa with diameter of 150um and skin cell with diameter of 1500 um (have to be
greater than 100um
Label the following unmarked microscope components by matching it with the
components provided
1. Eyepiece
2. Neck
3. Fine adjustment knob
4. Objective
5. Stage
6. Base
, This type of microscope is best suited for visualizing GFP, RFP and YFP proteins
(given list of microscopes)
Flourescence
This type of microscope can provide detailed images of live cells without staining
(given list of microscopes)
Phase-Contrast
This type of microscope is used to greatly increase the contrast between samples
and background by reflecting light off of the specimen (given list of microscopes)
Dark Field
This type of microscope is capable of capturing images in multiple focal planes
rendering specimen in 3D (given list of microscopes)
Confocal
Identify what type of electron microscope was used to capture the following
image and explain your choice
A: TEM, shows 2-D rendering
B: SEM shows 3-D shell models
Gram-Positive cells appear _____ in color due to a _____ peptidoglycan layer in
the cell wall (fill in the blanks)
Purple, thick
Gram-Negative cells appear _____ in color due to a _____ peptidoglycan layer in
the cell wall (fill in the blanks)
Pink, thin
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