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TAMU ANSC 333 Lab Quiz - Assisted Repro Technologies Questions & answers
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TAMU ANSC 333 Lab Quiz - AssistedRepro Technologies
in vitro - ANS-"in the glass, or dish"
IVF (In Vitro Fertilization) - ANS-Which repro technology involves "placing a sperm and
mature oocyte together in a dish for fertilization."
morula or blastocyst - ANS-Embryos are commonly kept in vitro until they mature to
____ or ____ stage, before being frozen or transferred. This is referred to as an in vitro
culture (IVC), or in vitro maturation (IVM).
sperm, oocyte, and somatic cells surrounding the oocyte (cumulus cells) - ANS-For IVF,
the media must be suited for which cell(s)?
Aspiration of the oocytes
Mature the oocytes to Metaphase II
Change media and add sperm (left alone to fertilize themselves, not with technology)
Fertilization occurs
Culture for *7 days*, until morula or blastocyst
Freeze or transfer immediately. - ANS-What is the process of IVF?
Prophase I (Meiosis) - ANS-At which stage of embryonic development is aspiration for
IVF done?
2mm - ANS-How big (in diameter) are the follicles aspirated for IVF?
35-45% - ANS-From IVF, what % of mature ova produce embryos?
35-45% - ANS-What is the pregnancy rate of IVF embryos?
20-40% - ANS-What is the calving rate (%) from IVF pregnancies?
5-40% - ANS-What is the pregnancy rate range for IVF embryos *frozen*
pregnancy rates are unpredictable. - ANS-Though freezing has gotten better for IVF,
what is still a challenge?
, ICSI (intracytoplasmic sperm injection) - ANS-Which assisted repro tech. is relatively
similar to IVF, except a single sperm is injected into an oocyte mechanically?
use of genetically valuable sperm with poor motility, acrosomal defects, etc., for
fertilization. - ANS-What does ICSI enable?
undamaged DNA - ANS-What is the main requirement for sperm used in ICSI?
the cumulus cells surrounding the oocyte - ANS-Prior to ICSI, what is removed via
*enzymatic digestion*?
The oocyte (with no cumulus cells) is held using a holding pipette, and the sperm is
injected through the zona pellucida and oocyte membrane, and *into the cytoplasm* for
syngamy to occur. - ANS-Describe *how* the sperm is injected into the oocyte for ICSI.
Gamete Intrafollopian Transfer - ANS-What does GIFT stand for?
surgically or laparoscopically transferring male and/or female gametes into the oviduct
(fallopian tube). - ANS-What is the GIFT process?
to artificially inseminate with sperm that have transport problems, allows sperm to
bypass cervix and uterus. - ANS-Why is GIFT primarily used?
ampullary-isthmic junction - ANS-GIFT allows sperm to bypass the cervix and uterus.
Where do they get deposited directly into?
GIFT - ANS-Which assisted reproductive technology may be used to replace IVF?
many ova and sperm can be placed in one recipient oviduct for fertilization.
the embryos may be flushed then transferred to numerous recipients
embryos freeze better than those created by IVF - ANS-Why may GIFT be used to
replace IVF?
Somatic Cell Nuclear Transfer (SCNT) - ANS-What does SCNT stand for?
to create genetically identical animals or for the creation of embryonic stem cells.\ -
ANS-What is SCNT used for?
embryo splitting - ANS-Prior to *SCNT*, people were creating clones how?
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