Protein Engineering Exam Questions With Verified Answers.

Protein Engineering Exam Questions With Verified Answers. Why create diversity? - answer•Control evolution -Speeding up the natural randomness -Focusing changes •Refine creations -Post screening enhancements -Locating unknown fitness apex Random mutagenesis (PCR-based) - answerRequirements: •Low-fidelity polymerase •Degenerative primers •"Mimics" nature •No knowledge needed! Random mutagenesis (non-PCR-based) - answer•Transposons insert themselves within DNA -Random or targetted •Can be used to insert or delete DNA Site directed mutagensis - answerRequirements: •Knowledge of sequence + structure •Understanding of mechanism •Know what you want to do! CRISPR-Cas - answer•(Clustered regularly interspaced short palindromic repeats) •Guiding RNA (gRNA) used to target area •Cas9 protein used to cut DNA •Potential use of "repair fragment" to insert genetic material