Summary
Summary on recombinant DNA technology
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This document is about rDNA technology and vectors used for this.
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Section 7: Recombinant DNOther Tools in Biote
Restriction enzyme, a protein produced by bacteria that
molecule. In the bacterial cell, restriction enzymes cleave
organisms. Restriction enzymes can be isolated from bac
to manipulate fragments of DNA, such as those that co
indispensible tools of recombinant DNA techn
A bacterium uses a restriction enzyme to defend against b
or phages. When a phage infects a bacterium, it inserts i
, Each restriction enzyme recognizes a short, specific seque
bases (the four basic chemical subunits of the linear doub
molecule—adenine, cytosine, thymine, and guanine). Th
called recognition sequences, or recognition sites, and
distributed throughout the DNA. Different bacterial s
restriction enzymes that recognize different nucleotid
When a restriction endonuclease recognizes a sequence,
the DNA molecule by catalyzing the hydrolysis (splittin
bond by addition of a water molecule) of the bond bet
nucleotides. Bacteria prevent their own DNA from being
manner by disguising their recognition sequences. En
methylases add methyl groups (—CH3) to adenine or cyto
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