PATHOLOGIE
1. Rol van de hematopatholoog in de diagnostiek van hematologische aandoeningen
1.1. Anatomie en fysiologie van de lymfoïde weefsels
- Centrale vs perifere lymfoïde weefsels
- Primaire vs secundaire lymfoïde weefsels
- CD-merkers
1.2. Rol van de hematopatholoog in het diagnostisch proces
1.2.1. Conventionele pathologie: classificatie: karakterisering van maligne cellen
- Verdere investigatie: relatief vs neoplastisch
- Prelevatie van het juist staal: beenmerg en lymfoïd weefsel (excisie vs CNB vs FNAC)
- Klassiek lichtmicroscopisch onderzoek: architectuur, cytologisch onderzoek en
immunohistochemisch onderzoek
- Aanvullende moleculair-genetisch onderzoek (wkn): PCR vs FISH vs NGS
1.2.2. Biomerker detectie
- Expressie van biomerkers in/op tumorcellen
- Samenstelling van tumor-micromilieu bepaalt prognose
1.2.3. Therapeutic decision making: targeted therapy
- Monoclonale antilichamen
- Opsporen van targetable mutations dmv NGS (GCB en ABC)
2. Reactieve lymfadenopathies: Aandoeningen van het lymfoïd weefsel
2.1. Inleiding
- Reactieve processen vs tumorale processen
- Microarchitectuur van een lymfeklier
2.2. Folliculaire hyperplasie (B-cellen)
- Primaire B-follicle vs Secondary B-follicle
- Casus: Kind, 4j, warm, rood, pijnlijk gezwollen cervicale lymfeklieren
◦ Reactieve aandoeningen gelokaliseerd ter hoogte van de B-follikel of folliculaire
hyperplasie
◦ Morfologische kenmerken
◦ Functionele betekenis
◦ Differentiaal diagnose: folliculair lymfoom
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