MCB3020 Exam 3 Bacusmo Questions And Answers 100% Correct
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Course
MCB 3020
Institution
MCB 3020
MCB3020 Exam 3 Bacusmo Questions And
Answers 100% Correct
Biotechnology use of organisms to form useful products
Genetic engineering the deliberate modification of the organisms genetic information by directly
changing the sequence of nucleic acids in its genome
Recombinant DNA technology pr...
MCB3020 Exam 3 Bacusmo Questions And Answers 100% Correct Biotechnology use of organisms to form useful products Genetic engineering the deliberate modification of the organisms genetic information by directly changing the sequence of nucleic acids in its genome Recombinant DNA technology procedures used to carry out genetic engineering Cloning generation of a large number of identical DNA molecules 5 basic steps of cloning 1. isolate DNA to be cloned 2. Use restriction enzyme or PCR to generate fragments of DNA 3. Generate recombinant molecule by inserting DNA fragments into a cloning vector 4. Introduce recombinant molecule into new host which expresses the gene 5. culture host gene, making identical copes restriction enzymes endonucleases that recognize and cleave specific DNA sequences T/F: Restriction enzymes sometimes make staggered cuts, which produce single -stranded DNA sequences known as "sticky ends". true What enzyme forms covalent bonds between the cloned gene and the plasmid, creating recombinant DNA? ligase reverse transcriptase enzyme that synthesizes double stranded DNA from RNA template (mRNA) MCB3020 Exam 3 Bacusmo Questions And Answers 100% Correct What is the process for constructing cDNA? What is the goal of this process? Processed mRNA is taken from eukaryotic cell, poly dT primer is added, reverse transcriptase and dNTPs are added to synthesize complementary DNA strand, RNaseH digests mRNA to make primers needed to synthesize second DNA strand, exonuclease removes RNA pri mers, cDNA ready to be inserted; make DNA from RNA template What is gel electrophoresis? Why is it important? used to separate molecules based on their charge and molecular weight; weight of specific DNA sequences can be measured by comparison to a ladder - used in DNA fingerprinting Nucleic acids have _ charge due to their __ __ (2 words), so they migrate through the electric field towards the _ electrode. negative; backbone phosphates; positive True or False: Shorter DNA will travel farther in gel electrophoresis because smaller restriction fragments move faster. True What dye is used to stain DNA? ethidium bromide what is PCR? in vitro technique used to synthesize large quantities of specific nucleotide sequences from a small amount of DNA; employs oligonucleotide primers complementary to specific sequences in the target gene and heat stable DNA polymerases taq polymerase A heat -stable form of DNA polymerase extracted from bacteria that live in hot environments, such as hot springs, that is used during PCR technique What are the 4 things required in PCR? template to copy, primers for free 3' -OH, 4 dNTPs, taq polymerase MCB3020 Exam 3 Bacusmo Questions And Answers 100% Correct True or False: In PCR, hydrogen bonds of double -stranded DNA molecules are broken by subjecting the DNA to high levels of radiation. False, under high levels of heat If you perform 6 cycles of PCR on a single double -stranded DNA molecule. How many copies will you have? 64 cloning vector Plasmids that can carry foreign DNA into a host cell and replicate there. What are the 3 things every "good" cloning vector must have? origin of replication, region of DNA that bears unique restriction sites (multicloning site), selectable marker plasmids small circular DNA molecules that replicate separately from the bacterial chromosome; most commonly used, only in prokaryotes bacteriophages genetically modified phage genomes that include restriction enzyme recognition sites for insertion cosmids vector with lambda phage cos sites that can be packaged into a phage capsid; useful for large amounts of DNA artificial chromosomes Used when large fragments of DNA must be cloned; also only replicate once per cell cycle genomic library complete collection of cloned DNA fragments from an organism cDNA DNA copy of an RNA molecule
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