LS23L |232 Final Study Guide Quiz Questions|2024 __ cells are striated, tubular, and multi -nucleated. They are classified as voluntary and their structure allows for locomotion and movement. - ✔ ✔ skeletal muscle 1 microgram (μg) is equal to how many milli grams (mg)? - ✔ ✔ 0.001 A class is studying b -galactosidase activity in a bacterial culture. After a student adds ONPG to their sample and incubates for 10 minutes, the student then adds 10 uL PopCulture, mixes the samples, and allows the samples to sit br iefly before taking an enzymatic assay reading using a spectrophotometry. To the student's surprise, their enzymatic assay reading was significantly lower than the rest of the class's. What is the best explanation for this? - ✔ ✔ The student added ONPG to the bacterial cells before lysing the cells. This does not allow the b -galactosidase to come into contact with ONPG during the incubation period. A class is studying b -galactosidase activity in a bacterial culture. After a student adds ONPG to their sample , the student adds two drops of PopCulture Reagent, mixes the samples, and allows the samples to sit before transferring the supernatant to a cuvette for a enzymatic assay reading using a spectrophotometry. To the student's surprise, their enzymatic assay reading was significantly lower than the rest of the class's. What is the best explanation for this? - ✔ ✔ The student added ONPG to the bacterial cells before lysing the cells. This does not allow the b -galactosidase to come into contact with ONPG, which may have also been damaged by its direct exposure to PopCulture Reagent. A life science student uses a compound microscope to look at a slide of human tissue. He is not sure what part of the body the tissue is from, but makes an educated guess based on the cells he sees on the slide. There are hundreds of round disks with no nucleus, as well as a couple of purple -colored disk -like cells. The student would most likely guess that the tissue is of.....? - ✔ ✔ Blood A null hypothesis states that the difference between 8 hours of sleep and 3 hours of sleep has no effect on the number of pictures a student will remember during a Picture Memory Interface Test (PMIT). Examining the MIT database, an extremely sma ll t-value of 0.1611 is found. This t -value is much smaller than the smallest t -value on the chart for p -value reference. From this information we can: - ✔ ✔ Accept the null hypothesis because the p -value obtained shows that the difference between the two groups being tested is not statistically significant A p-value of 0.04 obtained from a two -tailed t -test means that there is a ____ % chance that the obtained results are due to _______, assuming that the _________ hypothesis is correct. - ✔ ✔ 4, chance, n ull A p-value of 0.04 obtained from a two -tailed t -test means that there is a ____ % chance that the obtained results are due to _______. Given the conventional significance level, you would accept that the _________ hypothesis is correct. - ✔ ✔ 4, chance, alternative A scientist runs an SDS -PAGE experiment two separate times on a protein with a molecular weight of 200 kD. For the first experiment he treats the protein with SDS only and for the second experiment he treats the protein with both SDS and a red ucing agent. If the first gel yields one band showing a molecular weight of 200 kD and the second run yields one band showing a molecular weight of 25 kD, the researcher should conclude that: - ✔ ✔ The consists of 8 subunits of equal size connected by a disulfide linkage. A student conducts a metabolism experiment with goldfish in an oxygen chamber. With the chamber filled, the student notices bubbles (3mm in diameter) inside of the chamber befor e starting the experiment. What should the student do to obtain the most accurate results? - ✔ ✔ Open the chamber and close it again to remove all of the air bubbles, then proceed A student wants to count the number of chloroplasts in a living plant cell. What kind of microscope should she use? - ✔ ✔ A compound microscope A student was trying to identify three slides. The first slide consisted of a cluster of cells that contained no nuclei. The second slide consisted of cells that were very densely packed w ith nuclei. The third slide consisted of cells that were porous in places, with nuclei that were spread out. What did the three slides depict? a. The three slides depicted red blood cells, thymus, and bone tissue, respectively. b. The three slides depict ed liver, red blood cells, and neurons, respectively. c. The three slides depicted kidney, liver, and bone tissue, respectively. d. The three slides depicted thymus, kidney, and red blood cells, respectively. e. The three slides depicted red blood cells, l iver, and neurons, respectively. - ✔ ✔ A. The three slides depicted red blood cells, thymus, and bone tissue, respectively. A volume of liquid measuring __ is most accurately measured and dispensed using a __ pipetter. - ✔ ✔ 0.12 ml, p200 A____ p -value and a____ t -test value indicate that the null hypothesis is____ likely to be true. - ✔ ✔ lower, lower, less According to the equation that was created from the standard curve in SDS -PAGE lab, as the x -value ___, the y -value ___. - ✔ ✔ increases, decreases According to the Lab Safety Sheet provided for this week, when working with E. coli cells you must wear: (check all that apply) - ✔ ✔ Safety goggles, standard lab attire, lab coat, and gloves. According to the lab safety sheet, during the epidemiology lab act ivity, you must wear: - ✔ ✔ standard lab attire, safety goggles, lab coat, and a glove on your non -dominant hand According to the lab safety sheet, the water used for the fish is treated with all of the following chemicals except except: - ✔ ✔ EDTA Accordi ng to the Research Information Sheet, possible risks you assume while participating in the Memory Interference Test (MIT) include: - ✔ ✔ Fatigue/tiredness According to the Research Information Sheet, what is a benefit to subject and/or society from partici pating in this study? - ✔ ✔ You will help develop better ways to evaluate cognition in people After completing LS3, you decide you love the class so much, you want to UA for it. During the Sequence Analysis and Maternal Lineages lab you notice something od d with your results. When you took LS3, you had been placed in haplogroup F, but this time you were placed in haplogroup N. What is the most probably cause of this discrepancy? - ✔ ✔ During one run you had a shortened sequence (about 200 bp) while the othe r you had a good quality sequence (about 400 bp) After performing a gel electrophoresis with sample proteins that have multiple subunits, John noticed that all his protein samples moved down the gel but there was only one band in each of his gel lanes when there was supposed to be more than one. He was confused because he even added denaturing detergent to his samples. What could have been a mistake John made? - ✔ ✔ John forgot to heat his protein samples to denature them All are true of a compound light microscope except which of the following: a. It focuses on light that reflects off the surface of the specimen. b. It requires a thin specimen. c. It has discret e increments of magnification. d. It produces a mirror image. e. It contains two lenses. - ✔ ✔ a. It focuses on light that reflects off the surface of the specimen. All of the following are characteristics of gel electrophoresis except: a. has small pores that allow detection of smaller molecules
