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Samenvatting Campbell and Reece: H18
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Samenvatting van H18 uit het boek Campbell and Reece: biology. Handig voor deeltoets 2 van het vak moleculaire biologie!
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HOOFDSTUK 18: CONTROL OF GENE EXPRESSIONConcept 18.1
Tryptofaan synthese regulatie
Alleen metabole pathway actief als er geen tryptofaan aanwezig is
Regulatie op 2 niveaus:
● Enzym regulatie
○ Direct
○ Feedback inhibitie
● Transcriptie, expressie van genen
○ Operon model
Operon codeert verschillende genen voor 1 metabole pathway
2 vormen:
1. Repressible operon
○ Trp-operon
○ Transcriptie wordt geremd door de repressor gebonden aan de co-repressor
(tryptofaan)
2. Inducible operon
○ Lactose of Lac-operon
○ Transcriptie wordt geïnduceerd door de inducer
1
, Trp-operon model
● Een lang mRNA (polycistronisch)
○ Meerdere open leesramen
Tryptofaan afwezig
● Repressor inactief
Geen tryptofaan, repressor inactief, operon aan
Tryptofaan aanwezig
● Repressor bindt tryptofaan (de co-repressor)
● Repressor is actief en bindt aan de operator
● RNA-polymerase wordt geblokkeerd
Tryptofaan aanwezig, repressor actief, operon uit
2
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