Alles wat je moet weten voor de praktijk toets microbiologie VL4
- Alle 16 stammen beschreven
- Alle determinatie testen beschreven
- Zelf gemaakte foto's (macroscopisch en microscopisch)
Cijfertoets: 8,1
McC
- Lactose – en pigment (vegroening)
Determinatie: Groei op MC bleek oxidase uitvoeren: oxidase test + Api 20 NE inzetten of oxidase test -
Api 20 E inzetten
Gram:
- Gram negatief
- Staaf
- Los liggend
, Proteus mirabilis
Kolonie op bloedagar:
- Grijs/wit
- Zwermt in concentrische ringen (flagellen)
- Specifieke faecale geur
- Door de hoge pH wordt de plaat donker
- Geen hemolyse
MacConkey:
- Lactose –
- Niet zwermend
Determinatie: Groei op MC bleek oxidase uitvoeren: oxidase test + Api 20 NE inzetten of oxidase test -
Api 20 E inzetten
Gram:
- Gram negatief
- Staaf (klein)
- Los liggend
Haemophilus influenza
Groeit niet op BA of McC.
Chocolade plaat:
- Klein, waterige, grijze kolonie
- Geur: weeïg
Determinatie: Geen groei op MC groei alleen op Choc en verdacht voor Heamophilus X, V, XV test inzetten
Gram:
- Gram negatief
- Staafjes cocoid
- Los liggend
Bacillus cereus
Kolonie op bloedagar:
- Groot
- Plat
- Dof
- Grijs
- Soms β hemolyse
- Zwermend
Determinatie: Bonte rij of Api 20 E inzetten
Gram:
- Gram positief
- staven
- Ketens
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