Chemical Imaging Using Molecular Spectroscopy – Providing Answers to Fundamental Biomedical Questions at the Nanoscale.
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Course
Chemical Imaging
Institution
Chemical Imaging
Raman spectral imaging
The first step of Raman spectral imaging is to record a
Raman map. There are roughly three hardware approaches
in recording Raman maps – point mapping, line scanning
and global imaging.
Point mapping is the most often used method in the
industry because Raman...
Chemical Imaging Using Molecular Spectroscopy – Providing Answers to Fundamental Biomedical Questions at the Nanoscale. A. Whitley*, A. Gilmore*, E. Lee* and F. Adar* *HORIBA Jobin Yvon Inc., 3880 Park Avenue, Edison, NJ USA 08822, Eunah.Lee@jobinyvon.com ABSTRACT Recent and future major developments in molecular and micro-analysis for nanomaterial and biomedical applications will be discussed and explained in terms of our vision of how instrumentation will need to perform and operate in the future to meet the challenging measurements required for nanomaterial research and development. Keywords : Raman, AFM, SEM, spectral imaging, biomolecular imaging INTRODUCTION As leaders in Raman and fluorescence spectroscopy for over two decades HORIBA Jobin Yvon have been at the cutting edge of major breakthroughs in many key areas of instrument development and materials analysis. The history and philosophy of our instrument design including the latest hybrid approach of combining two or more techniques in one instrument for enhanced material characterization will be discussed. These hybrid combined microanalysis techniques include Raman/AFM, Raman/SEM, Raman/FT-IR and Raman/PL/CL. Recent instrumental advances have allowed biomolecular imaging to probe molecular properties of biological materials at the nanoscale. Biomolecular imaging simultaneously combines nanomaterial distribution, morphology and size measurements with molecular identification and conformation – in other words it answers most of your material characterization questions at the same time. This fundamental understanding of what determines the physical and chemical properties of biomaterials at the nanoscale provides researchers with the capability for true “intelligent design”. These breakthrough studies include direct imaging of cells without the need for staining or tagging (which will often result in missed information or misinformation), the determination and/or the prediction of the nanostructure failure mechanism in bone and artificial joints and the characterization of single-walled nanotube diameter and chirality. The significance and direct applicability of this type of research is becoming thoroughly established in the biomedical, material and physical sciences. With the concentration of research in nanotech, biotech and new energy alternatives – much of which in the future will also be directed towards nanotechnology, it is the intention HORIBA Jobin Yvon to continue to develop suitable instrumentation and software to enable and accelerate this research. Already we have developed or are developing instrumentation to allow Raman spectroscopy to provide direct molecular imaging of cancer, the location and monitoring of drug activity within tissue and at the celluar level, in-vivo spectroscopic guidance during surgery, in-situ studies during electronic and other physical perturbations of single nanotubes and drug interaction and delivery when combined with nanotubes. INSTRUMENTATION AND APPLICATIONS Raman microscopy is a coupled device of Raman spectrometer and a microscope, providing molecular identification and information about inter and intra-
molecular interactions and changes at the spatial resolution of the microscope. The microscope, ultimately, can be any type – e.g. an optical microscope or a scanning microscope – with unique advantages for each type. Automated Raman microscope A Raman microscope with an optical microscope is a well established technology and available commercially from multiple manufacturers (Figure 1). The spatial resolution can reach 200 nm, depending on the excitation laser wavelengths and the objective lens. Confocality is one of the unique advantages of an optical microscope enabling axial discrimination. Depth profiling of a transparent multi-layer sample (e.g. polymer) provides Figure 1. LabRAM ARAMIS IR2, a commercially available, fully automated Raman microscope combined with an optical microscope and FT-IR
NSTI-Nanotech 2007, www.nsti.org, ISBN 1420061836 Vol. 2, 2007339
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