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Microbiology UTA - Lab Practical 1 with Complete Solutions
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Microbiology UTA - Lab Practical 1 with Complete Solutions
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Microbiology UTA - Lab Practical 1 withComplete Solutions
A photo is listed as having a total magnification of 400x. Which of the following
objectives was used in order to take the photo with a microscope that had 10x oculars...
- ANS-the 40x objective
Adjusting the amount of light that enters the substage condenser allows greater control
of contrast of the specimen being observed. This is controlled by adjusting the field
diaphragm lever... - ANS-false
Compound microscopes are able to maintain proper focus of a specimen when moving
from low power to higher power magnification due to a property known as... - ANS-
parfocal
Gram staining allows determination of the species of bacteria in a sample... - ANS-false
How do you express 1/100 in scientific notation... - ANS-10^-2
Identify the structure indicated by the red arrow... - ANS-field diaphragm lever
Identify the structure indicated by the red arrow... - ANS-fine focus adjustment knob
If you make a 1/100 dilution in your first sample and then use that to make a 1:10
dilution into your second sample, what is the dilution in your second sample... - ANS-
1/1000 (10^-3)
If you transfer bacterial growth from a broth to an agar plate but nothing grows after
incubation, what may have occurred... - ANS-The loop may have been too hot when
making the transfer
Indicate the correct order of steps for a broth to agar plate transfer...
1. Lift the lid of the agar plate slightly and streak the loop across the surface of the agar
plate. Replace the lid.
2. Remove a loopful of bacterial culture from the broth tube, pass the mouth of the tube
through the Bunsen burner flame, and replace the lid.
3. Sterilize inoculating loop prior to replacing in loop holder.
4. Sterilize inoculating loop, remove cap of culture tube, and pass mouth of culture tube
through the Bunsen burner flame. - ANS-4, 2, 1, 3
Indicate the correct order of steps for a broth to broth transfer...
1. Sterilize and cool inoculating loop, remove cap of culture tube, pass mouth of tube
through Bunsen burner flame.
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