Exam (elaborations)
Microbial Genetics and Genetic Engineering Straigherline Lab with Answers
- Course
- Institution
Complete lab with answers
[Show more]
Seller
Follow
Content preview
Lab 7 Microbial Genetics & Genetic Engineering BIO250L”Student Name: xxxx
Access Code (located on the underside of the lid of your lab kit): xxxxx
“Pre-Lab Questions”
1. Which DNA nitrogenous bases pair with each other? Which bases are purines, and which are
pyrimidines?
DNA nitrogenous base pairs with each other are:
Adenine and Guanine are purines
Cytosine and Thymine are pyrimidines
2. How is DNA information used to make proteins? What are the steps of this process?
DNA is used to make protein through the process of transcription and translation. When
enzymes from DNA molecules are transcribed into mRNA with RNA polymerases. The
next mRNA reaches the ribosome, then tRNA and translates into amino acids that block
proteins.
3. Give an example of a scenario in which you would perform PCR vs a scenario in which you would
use recombinant DNA technology.
You would perform PCR when determining an illness and would be used to get DNA
from a blood sample. When using recombinant DNA technology it can be used for
human growth hormones such as insulin.
4. What occurs during each of the three steps involved in the PCR cycle? How has the use of PCR
changed biotechnology?
The three steps (parts) to the PCR cycle are: Denaturing, Annealing, and Extending.
During denaturing the mixture is heated to separate the DNA strands. Next is the
annealing phase where it is cooled enough to facilitate hydrogen bonds to form new
separated DNA strands. Lastly the temperature is raised again to allow DNA polymerase
to use the primer-DNA hybrids to synthesize two new DNA molecules. PCR has
changed biotechnology, in that now many viral and bacterial infections can now be
identified with PCR screenings.
5. How could you take a protein with a known sequence of amino acids and use it to create an
artificial gene?
A protein with a known sequence of amino acids can be used to create an artificial gene
by using recombinant DNA technology with bioinformatic tools.
“EXPERIMENT 1: DNA Extraction
Post-Lab Questions
1. What is the purpose of the following reagents in the experiment?
The benefits of buying summaries with Stuvia:
Guaranteed quality through customer reviews
Stuvia customers have reviewed more than 700,000 summaries. This how you know that you are buying the best documents.
Quick and easy check-out
You can quickly pay through credit card or Stuvia-credit for the summaries. There is no membership needed.
Focus on what matters
Your fellow students write the study notes themselves, which is why the documents are always reliable and up-to-date. This ensures you quickly get to the core!
Frequently asked questions
What do I get when I buy this document?
You get a PDF, available immediately after your purchase. The purchased document is accessible anytime, anywhere and indefinitely through your profile.
Satisfaction guarantee: how does it work?
Our satisfaction guarantee ensures that you always find a study document that suits you well. You fill out a form, and our customer service team takes care of the rest.
Who am I buying these notes from?
Stuvia is a marketplace, so you are not buying this document from us, but from seller amandamschiebel. Stuvia facilitates payment to the seller.
Will I be stuck with a subscription?
No, you only buy these notes for $3.99. You're not tied to anything after your purchase.
Can Stuvia be trusted?
4.6 stars on Google & Trustpilot (+1000 reviews)
74735 documents were sold in the last 30 days
Founded in 2010, the go-to place to buy study notes for 14 years now