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Exam (elaborations)

ASCP MB 2024 VERIFIED QUESTIONS AND ANSWERS

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  • ASCP MB

E. coli RNA Polymerase II - CORRECT ANSWER DNA template, product: mRNA RNA Polymerase I - CORRECT ANSWER DNA template Location: Nucleous Products: 18s, 5.8s, 28s rRNA RNA Polymerase II - CORRECT ANSWER DNA template Location: Nucleus Products: mRNA, snRNA R...

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  • August 4, 2024
  • 51
  • 2024/2025
  • Exam (elaborations)
  • Questions & answers
  • ascp mb 2024
  • ascp mb
  • ASCP MB
  • ASCP MB
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ASCP MB 2024 VERIFIED QUESTIONS AND
ANSWERS
E. coli RNA Polymerase II - CORRECT ANSWER DNA template,
product: mRNA


RNA Polymerase I - CORRECT ANSWER DNA template
Location: Nucleous
Products: 18s, 5.8s, 28s rRNA


RNA Polymerase II - CORRECT ANSWER DNA template
Location: Nucleus
Products: mRNA, snRNA


RNA Polymerase III - CORRECT ANSWER DNA template
Location: Nucleus
Product: tRNA, 5s rRNA, snRNA


Mitochondrial RNA Polymerase - CORRECT ANSWER DNA template,
product: mRNA


Mammalian DNA polymerase α - CORRECT ANSWER DNA template,
product: primers


HCV RNA Polymerase - CORRECT ANSWER RNA template, product:
viral genome

,ASCP MB 2024 VERIFIED QUESTIONS AND
ANSWERS
Dengue virus RNA Polymerase - CORRECT ANSWER RNA template,
product: viral genome


Poly A Polymerase - CORRECT ANSWER No template, product: Poly A
tail


230 wavelength - CORRECT ANSWER Contamination of organic
compounds


270 wavelength - CORRECT ANSWER Phenol contamination


280 wavelength - CORRECT ANSWER Protein contamination


>330 wavelength - CORRECT ANSWER Particulate matter
contamination


Southern Blot - CORRECT ANSWER DNA Target
Nucleic acid probe
Purpose: Paternity testing, gene discovery and mapping, mutation analysis
and ID, gene structure


Northern Blot - CORRECT ANSWER RNA Target
Nucleic acid probe
Purpose: Determine transcript structure, processing, and gene expression

,ASCP MB 2024 VERIFIED QUESTIONS AND
ANSWERS

Western Blot - CORRECT ANSWER Protein Target
Protein probe
Purpose: Protein processing, gene expression


Southwestern Blot - CORRECT ANSWER Protein target
DNA probe
Purpose: DNA binding proteins, gene regulation


Eastern blot - CORRECT ANSWER Protein target
Protein probe
Purpose: Modification of Western blot using enzymatic detection (protein
processing, gene expression), detection of specific agriculturally important
proteins


Far-eastern blot - CORRECT ANSWER Lipid target
No probe
Purpose: Transfer separated lipids to membranes for analysis by mass
spectometry


PCR Primers - CORRECT ANSWER Direct DNA synthesis to desired
region

, ASCP MB 2024 VERIFIED QUESTIONS AND
ANSWERS
PCR: dATP, dCTP, dGTP, dTTP - CORRECT ANSWER Building blocks
that extend primers


PCR KCl - CORRECT ANSWER Salt for optimal hybridization of
primers to template


PCR: Tris buffer - CORRECT ANSWER Buffer to maintain optimal pH
for enzyme reaction


PCR: MgCl2 - CORRECT ANSWER Divalent cation, required by enzyme


PCR Polymerase - CORRECT ANSWER Polymerase enzyme that
extends the primers, adds the dNTPs


PCR: copies of template - CORRECT ANSWER Sample DNA that is
being tested


Denaturation temps/time - CORRECT ANSWER 90-96, 20-60 sec


Annealing temps/time - CORRECT ANSWER 50-70, 20-90 sec


Extension temps/time - CORRECT ANSWER 68-75/10-60 sec


+- CORRECT ANSWER gain

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