Micro 2460 UTA AOBSN Lab Practical 1 (Labs 1, 2, 3, 4)
### Components of a Light Microscope
1. Ocular/Eyepiece: - Typically has 2 eyepieces for binocular viewing. - Commonly provides 10x magnification. - Adjusts in and out to provide comfortable viewing. - When positioned correctly, produces a ...
- Typically has 2 eyepieces for binocular viewing.
- Commonly provides 10x magnification.
- Adjusts in and out to provide comfortable viewing.
- When positioned correctly, produces a single circle of light.
2. Body:
- Holds mirrors that bend light into the oculars.
- The image seen through the ocular is reversed and inverted.
3. Nosepiece:
- This part is where objectives are attached and can be rotated to switch between different
magnifications.
4. Objectives:
- These contain the magnifying lenses with different magnifications:
- 4x (lowest power)
- 10x (low power)
- 40x (high power)
- 100x (oil immersion)
5. Arm:
- Supports the nosepiece.
- Houses the power switch and focus adjustment knobs.
, 6. Stage:
- A flat platform that securely holds the mechanical stage.
7. Mechanical Stage:
- Holds the slide and allows for movement of the slide to properly position it under the objectives.
8. Stage Adjustment Knobs:
- These knobs move the mechanical stage, allowing adjustments in two directions: right and left,
forward and back.
9. Light Intensity Control:
- Used to increase or decrease the light output based on the needs of the specimen being observed.
10. Substage Condenser:
- Comprises two or more lenses that focus light as it passes through the specimen and enhances
image sharpness.
11. Aperture Diaphragm Control:
- Controls the amount of light entering the condenser.
- Should be 1/2 open for 10x and 40x magnification, and completely open for oil immersion.
- Too much light can bleach out the specimen.
12. Field Diaphragm Lever:
- Adjusts the intensity of light from the light source but should remain open during observation to
ensure proper illumination.
13. Light Source:
- Typically a halogen bulb that provides the necessary illumination for viewing samples.
14. Base:
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